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| Web URL(s): | http://archive.lib.msu.edu/tic/ressum/2009/9.pdf Last checked: 01/23/2017 Requires: PDF Reader |
| Publication Type: | Report |
| Author(s): | Lakshman, Dilip K. |
| Author Affiliation: | USDA-ARS |
| Title: | Accurate identification and gene expression in relation to virulence of Rhizoctonia isolates infecting turfgrasses |
| Section: | Integrated turfgrass management Other records with the "Integrated turfgrass management" Section |
| Source: | 2009 Turfgrass and Environmental Research Summary. 2009, p. 9. |
| Publishing Information: | Far Hills, NJ: United States Golf Association |
| # of Pages: | 1 |
| Keywords: | TIC Keywords: Disease identification; Gene expression; Rhizoctonia blight; Rhizoctonia cerealis; Rhizoctonia solani; Rhizoctonia zeae; Waitea circinata var. zeae |
| Abstract/Contents: | Presents a study conducted to determine the "molecular identification of Rhizoctonia solania isolates pathogenic to turfgrasses using Universally Primed-Polymerase Chain reaction (UP-PCR) and nucleic acid hybridization analysis." Describes the experiment and the methods used, explaining that "we collected a total of 448 Rhizoctonia samples from 5 locations in northern Virginia and Maryland during the summer of 2008 and 2009." |
| Language: | English |
| References: | 0 |
| See Also: | Other Reports from this USGA research project: 2007-10-351 |
| Note: | Pictures, b/w |
| USGA Summary Points: | We have collected a total of 448 Rhizoctonia isolates from golf courses and lawns of Virginia and Maryland. Analyzing the rDNA-ITS sequence variations of 54 randomly sampled isolates, we found that majority (i.e., 61%) of the isolates belong to R. solani, while 22% belong to R. zeae/W. cercinata var circinata, and 17% belong to R. cerealis/Rhizoctonia-like fungi. Of the identified R. solani isolates in this study, majority belong to AG2-2IIIB and AG 1-1B. Genome fingerprinting of Rhizoctonia isolates using UP-PCR and AFLP techniques are in progress. Analyzing the two virulence-differentiated EST libraries, we have identified 532 and 495 unigenes of R. solani AG-4. Among several indentified genes are thsoe with possible roles as elicitors of disease, in cell wall degradation, avoidance of host and drug resistance and mating. |
| ASA/CSSA/SSSA Citation (Crop Science-like – may be incomplete): | Lakshman, D. K. 2009. Accurate identification and gene expression in relation to virulence of Rhizoctonia isolates infecting turfgrasses. Turfgrass Environ. Res. Summ. p. 9. |
| Fastlink to access this record outside TGIF: | http://tic.msu.edu/tgif/flink/RECNO/159799 |
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| Web URL(s) : | http://archive.lib.msu.edu/tic/ressum/2009/9.pdf Last checked: 01/23/2017 Requires: PDF Reader |
| MSU catalog number: | b3609415 |
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