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| Full TGIF Record # 310367 Item 1 of 1 |
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| Web URL(s): | https://scisoc.confex.com/scisoc/2019am/meetingapp.cgi/Paper/122065 Last checked: 02/07/2020 Requires: JavaScript |
| Publication Type: | Report |
| Content Type: | Abstract or Summary only |
| Author(s): | Lu, Li Chieh; Del Valle, Ilenne; Masiello, Caroline A.; Silberg, Jonathan J. |
| Author Affiliation: | Lu: Biochemistry and Cell Biology, Rice University, Houston, TX; Del Valle and Silberg: Systems, Synthetic, and Physical Biology, Rice University, Houston, TX; Masiello: Earth, Environmental and Planetary Sciences, Rice University, Houston, TX |
| Title: | Chemical-sniffers: Detection of toxic chemicals in soils using synthetic microbes |
| Section: | C05 turfgrass science Other records with the "C05 turfgrass science" Section Turfgrass management and ecology poster (includes student competition) Other records with the "Turfgrass management and ecology poster (includes student competition)" Section |
| Meeting Info.: | San Antonio, Texas: November 10-13, 2019 |
| Source: | ASA, CSSA and SSSA International Annual Meetings. 2019, p. 122065. |
| Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America] |
| # of Pages: | 1 |
| Keywords: | TIC Keywords: Biological control; Chemical soil analysis; Microbial activity; Problem diagnosis; Soil microorganisms; Toxic soils; Toxic substances |
| Abstract/Contents: | "Soils can contain toxic chemicals as a result of various causes ranging from warfare to the leaching of fertilizers. Such chemicals often pose a threat to the wellbeing of the local populations. Exacerbating the problem is the fact that most techniques for detecting and removing such chemicals in the soil rely on analytical instruments which are often expensive to deploy and have difficulty providing real-time information about chemicals in locations other than the surface soil horizon. Furthermore, such instruments often provide information on the total extractable amount of a chemical, rather than the bioavailable concentrations. Synthetic biology offers a convenient alternative through the engineering of soil bacteria to sense and report on the presence of chemicals in different soil environments. Here we will discuss our efforts to develop a multi-input biosensor that communicates the sensing of an underground pollutant through a microbial community to the surface of a soil. Coupling of sensing and reporting is carried out by two component systems consisting of a chemical sensor protein and a response regulator that only activates gene expression when the chemical is sensed by the first protein. To enable directional cell-cell communication, we are developing a microbial strain that can receive the output of the first sensing strain and relay that information up a redox gradient in soil using a biological AND logic gate. We will describe our efforts in characterizing different methyl halide-synthesizing enzymes and methyl halide-dependent expressions systems so as to construct 'sender' and 'receiver' microbes. Additionally, we will describe how these microbes can be used in a safe laboratory setting to increase our fundamental understanding of signaling in soils." |
| Language: | English |
| References: | 0 |
| Note: | This item is an abstract only! "364" "Poster #1608" |
| ASA/CSSA/SSSA Citation (Crop Science-like – may be incomplete): | Lu, L. C., I. Del Valle, C. A. Masiello, and J. J. Silberg. 2019. Chemical-sniffers: Detection of toxic chemicals in soils using synthetic microbes. Agron. Abr. p. 122065. |
| Fastlink to access this record outside TGIF: | http://tic.msu.edu/tgif/flink/RECNO/310367 |
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| Web URL(s) : | https://scisoc.confex.com/scisoc/2019am/meetingapp.cgi/Paper/122065 Last checked: 02/07/2020 Requires: JavaScript |
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