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| Full TGIF Record # 324880 Item 1 of 1 |
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| Web URL(s): | https://scisoc.confex.com/scisoc/2022am/meetingapp.cgi/Paper/143525 Last checked: 03/01/2023 Requires: JavaScript https://issuu.com/leadingedgepubs/docs/va-turfgrass-2023-jan-feb/24 Last checked: 03/01/2023 Requires: JavaScript Notes: Virginia Turfgrass Journal reprint; Item is within a single large file |
| Publication Type: | Report |
| Content Type: | Abstract or Summary only |
| Author(s): | Tucker, Matthew; Eisenback, Jon D.; Haak, David; Henderson, Caleb A.; McCall, David S. |
| Author Affiliation: | Tucker: Presenting Author and Virginia Tech; Eisenback, Haak, Henderson, and McCall: Virginia Tech |
| Title: | A qPCR approach to lance nematode identification and quantification |
| Section: | Molecular Techniques, Genetics, Microbiome, and Turfgrass Breeding Oral (includes student competition) Other records with the "Molecular Techniques, Genetics, Microbiome, and Turfgrass Breeding Oral (includes student competition)" Section C05 turfgrass science Other records with the "C05 turfgrass science" Section |
| Meeting Info.: | Baltimore, Maryland: November 6-9, 2022 |
| Source: | ASA, CSSA, SSSA International Annual Meeting. 2022, p. 143525. |
| Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America] |
| # of Pages: | 1 |
| Abstract/Contents: | "Standard methods of lance (Hoplolaimus sp.) identification and quantification involve hand counting nematodes. These standard methods require time and a specific level of expertise to hand count both accurately and precisely. This is a subjective process that is time exhaustive when many samples need attention. The goal of this research is to evaluate if using a qPCR approach to identify and quantify Hoplolaimus sp. can be equivalent to the standard methods of nematode counting and more time efficient with bulk sampling. Samples were collected in September 2022 from Foundry Golf Club in Powhatan, VA (n=24). Traditional methods of nematode extraction and hand counting were performed to obtain Hoplolaimus sp. populations per sample. Each sample was saved in 25ml vials after counting for qPCR where both methods were compared. Hand counts showed 12.5% of samples were at or above the current Hoplolaimus sp. damage threshold of 100 nematodes per 100cc of soil. The average Ct value for the current economic threshold was determined to be 32.15 and any qPCR samples that are below that Ct value are considered problematic for golf course greens. Primers were selected based on former gene selection research for Hoplolaimus sp. (Bae et al., 2008) and specificity was tested against selected plant parasitic nematodes (PPN). Primer specificity assays of Hoplolaimus sp. alone exhibited a positive identification with an average Ct value of 36.56. All assays of Mesocriconema, Helicotylenchus, and Hemicycliophora alone were negative. Combinations of Hoplolaimus with each selected PPN above resulted in similar Ct values to Hoplolaimus alone ranging from 35.7 to 37.3. Overall, results with these methods show a level of success in amplification; however, these methods are inconsistent and currently would not be a viable alternative to traditional methods of nematode extraction." |
| Language: | English |
| References: | 0 |
| See Also: | See also related item, A qPCR Approach to Lance Identification and Quantification, 2022, R=343972.R=343972 |
| Note: | Reprint appears in Virginia Turfgrass Journal, January/February 2023, p. 24 This item is an abstract only! "47-11" |
| ASA/CSSA/SSSA Citation (Crop Science-like – may be incomplete): | Tucker, M., J. D. Eisenback, D. Haak, C. A. Henderson, and D. S. McCall. 2022. A qPCR approach to lance nematode identification and quantification. Agron. Abr. p. 143525. |
| Fastlink to access this record outside TGIF: | http://tic.msu.edu/tgif/flink/RECNO/324880 |
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| Web URL(s) : | https://scisoc.confex.com/scisoc/2022am/meetingapp.cgi/Paper/143525 Last checked: 03/01/2023 Requires: JavaScript https://issuu.com/leadingedgepubs/docs/va-turfgrass-2023-jan-feb/24 Last checked: 03/01/2023 Requires: JavaScript Notes: Virginia Turfgrass Journal reprint; Item is within a single large file |
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