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DOI: | 10.1002/agg2.20048 |
Web URL(s): | https://acsess.onlinelibrary.wiley.com/doi/10.1002/agg2.20048 Last checked: 05/04/2023 Requires: HTML5 https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.20048 Last checked: 05/04/2023 Requires: JavaScript; HTML5 https://acsess.onlinelibrary.wiley.com/doi/pdfdirect/10.1002/agg2.20048 Last checked: 05/04/2023 Requires: PDF Reader |
Publication Type: | Refereed |
Author(s): | Warnke, Scott E. |
Author Affiliation: | USDA-ARS U.S. National Arboretum, Floral and Nursery Plants Research Unit, Beltsville, MD |
Title: | Detection of interspecific hybrids between creeping bentgrass and colonial bentgrass using DNA marker analysis |
Section: | Agrosystems Other records with the "Agrosystems" Section |
Source: | Agrosystems, Geosciences & Environment. Vol. 3, No. 1, 2020, p. e20048 [1-7]. |
Publishing Information: | Madison, Wisconsin: American Society of Agronomy and Crop Science Society of America |
# of Pages: | 7 |
Abstract/Contents: | "Bentgrasses (Agrostis spp.) are cool-season turfgrasses that have utility on golf courses or other turf applications requiring low mowing heights. Interspecific hybridization of Agrostis species is a strategy that has been proposed to enhance biotic and abiotic stress tolerance in this genus. Non-transgenic, efficient, and cost-effective methods for interspecific hybrid detection are needed. In this study two bentgrass species creeping bentgrass (A. stolonifera L.), and colonial bentgrass (A. capillaris L.) were allowed to intercross under field conditions. Progeny plant DNA was extracted and stored using Flinders Technology Associates (FTA) PlantSaver cards followed by primer specific polymerase chain reaction (PCR) and high-resolution melt analysis. Species identifying PCR primers were optimized for real-time PCR through fragment resequencing of species-specific sequence characterized amplified region (SCAR) markers. A total of 2289 potential interspecific hybrid bentgrass progeny were screened with 99.4% of the DNA extractions successful. The percent interspecific hybridization, on an individual plant basis, ranged from 1 to 85% and a total of 524 interspecific-hybrid bentgrasses were identified representing 23% of plants tested. Interspecific hybridization is common between these bentgrass species and the proposed detection method provides a non-transgenic, efficient, and cost-effective method for interspecific hybrid identification." |
Language: | English |
References: | 19 |
Note: | Tables Graphs |
ASA/CSSA/SSSA Citation (Crop Science-like – may be incomplete): | Warnke, S. E. 2020. Detection of interspecific hybrids between creeping bentgrass and colonial bentgrass using DNA marker analysis. Agrosyst. Geosci. Environ. 3(1):p. e20048 [1-7]. |
Fastlink to access this record outside TGIF: | http://tic.msu.edu/tgif/flink/RECNO/327556 |
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DOI: 10.1002/agg2.20048 | |
Web URL(s) : | https://acsess.onlinelibrary.wiley.com/doi/10.1002/agg2.20048 Last checked: 05/04/2023 Requires: HTML5 https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.20048 Last checked: 05/04/2023 Requires: JavaScript; HTML5 https://acsess.onlinelibrary.wiley.com/doi/pdfdirect/10.1002/agg2.20048 Last checked: 05/04/2023 Requires: PDF Reader |
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