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| Full TGIF Record # 74459 Item 1 of 1 |
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| Web URL(s): | http://web.archive.org/web/20061205204057/http://www.uoguelph.ca/GTI/itsweb/proceedings.pdf#page=33 Last checked: 05/23/2017 Requires: Adobe Acrobat Notes: Document is within a single large file |
| Publication Type: | Report |
| Content Type: | Abstract or Summary only |
| Author(s): | Guo, Y. D.; Pulli, S. |
| Author Affiliation: | University of Turku |
| Title: | Somatic embryogenesis and plant regeneration from suspension cultures of timothy |
| Section: | Abstracts Other records with the "Abstracts" Section |
| Meeting Info.: | Toronto, Ontario, Canada: 15-21 July, 2001 |
| Source: | IXth International Turfgrass Research Conference. Vol. 9, 2001, p. 70. |
| Publishing Information: | [Toronto, Canada]: International Turfgrass Society |
| # of Pages: | 1 |
| Keywords: | TIC Keywords: Somatic embryogenesis; Regeneration; Phleum pratense; Calli; Inflorescences; Growth regulators |
| Abstract/Contents: | "As a graminaceous grass, timothy (Phleum pratense L. 2n=6x= 42) is one of the basic field crops in global food production, and the most important grass in northern latitudes. Timothy somatic embryogenesis and green plant regeneration were successfully established using immature inflorescences and seedling stems. The capacity of somatic embryogenesis from immature inflorescences was higher than that from seedling stems. Immature inflorescences of 0.5-1.5 cm in size cultured in MS medium containing 2 mg 1-1 2, 4-D gave the best performance in callus induction. Suspension cultures were initiated from friable nodule-forming callus in MS basal medium supplemented with 5.0 mg 1-1 2, 4-D and 0.5 mg 1-1 ABA. Kinetin at 1.0 mg 1-1 and 2,4-D at 0.1 mg 1-1 was the best plant growth regulator combination in green plant regeneration from direct callus differentiation and suspension cultures. The highest green plant regeneration was achieved 5-7 weeks after initiation in suspension culture and the rate of regeneration began to decline after 11 weeks of culture. More than 100 regenerated plants were obtained." |
| Language: | English |
| References: | 0 |
| See Also: | Other items relating to: P G Rs 1997-2006 |
| Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-like – may be incomplete): | Guo, Y. D., and S. Pulli. 2001. Somatic embryogenesis and plant regeneration from suspension cultures of timothy. Int. Turfgrass Res. Conf. 9:p. 70. |
| Fastlink to access this record outside TGIF: | http://tic.msu.edu/tgif/flink/RECNO/74459 |
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| Web URL(s) : | http://web.archive.org/web/20061205204057/http://www.uoguelph.ca/GTI/itsweb/proceedings.pdf#page=33 Last checked: 05/23/2017 Requires: Adobe Acrobat Notes: Document is within a single large file |
| MSU catalog number: | Uncataloged - Ask at TIC |
| Find from within TIC: | Digitally in TIC by file name: intrp2001 |
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