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DOI: | 10.1007/s10529-006-9162-z |
Web URL(s): | https://link.springer.com/article/10.1007%2Fs10529-006-9162-z Last checked: 10/04/2017 |
Publication Type:
| Refereed |
Author(s): | Hu, Qian;
Nelson, Kimberly;
Luo, Hong |
Author Affiliation: | Hu and Luo: Department of Genetics, Biochemistry and Life Science Studies, Clemson University, Clemson, South Carolina; Hu, Nelson, and Luo: HybriGene Incorporated, West Kingston, Rhode Island |
Title: | FLP-mediated site-specific recombination for genome modification in turfgrass |
Source: | Biotechnology Letters. Vol. 28, No. 22, November 2006, p. 1793-1804. |
Publishing Information: | [Kew, England: Science and Technology Letters] |
# of Pages: | 12 |
Keywords: | TIC Keywords: Agrostis stolonifera; Cell culture; Poa pratensis; Suspension cultures; Transgenes; Transgenic turfgrasses
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Abstract/Contents: | "To develop molecular strategies for gene containment in genetically modified (GM) turfgrass, we have studied the feasibility of using the FLP/FRT site-specific DNA recombination system from yeast for controlled genome modification in turfgrass. Suspension cell cultures of creeping bentgrass (Agrostis stolonifera L.) and Kentucky bluegrass (Poa pratensis) were co-transformed with a FLP recombinase expression vector and a recombination-reporter test plasmid containing β-glucuronidase (gusA) gene which was separated from the maize ubiquitin (ubi) promoter by an FRT-flanked blocking DNA sequence to prevent its transcription. GUS activity was observed in co-transformed cells, in which molecular analyses indicated that FLP-mediated excision of the blocking sequence had brought into proximity the upstream promoter and the downstream reporter gene, resulting in GUS expression. Functional evaluation of the FLP/FRT system using transgenic creeping bentgrass stably expressing FLP recombinase confirmed the observation in suspension cell culture. Our results indicate that FLP/FRT system is a useful tool for genetic manipulation of turfgrass, pointing to the great potential of exploiting the system to develop molecular strategies for transgene containment in perennials." |
Language: | English |
References: | 47 |
Note: | Pictures, color Figures Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Hu, Q., K. Nelson, and H. Luo. 2006. FLP-mediated site-specific recombination for genome modification in turfgrass. Biotechnol. lett. 28(22):p. 1793-1804. |
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| DOI: 10.1007/s10529-006-9162-z |
| Web URL(s): https://link.springer.com/article/10.1007%2Fs10529-006-9162-z Last checked: 10/04/2017 |
| MSU catalog number: b2322029 |
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