DOI: 10.1007/s10529-006-9162-z Web URL(s): https://link.springer.com/article/10.1007%2Fs10529-006-9162-z Publication Type:
i
Refereed Author(s): Hu, Qian ;
Nelson, Kimberly ;
Luo, Hong Author Affiliation: Hu and Luo: Department of Genetics, Biochemistry and Life Science Studies, Clemson University, Clemson, South Carolina; Hu, Nelson, and Luo: HybriGene Incorporated, West Kingston, Rhode Island Title: FLP-mediated site-specific recombination for genome modification in turfgrass Source: Biotechnology Letters . Vol. 28, No. 22, November 2006, p. 1793-1804.# of Pages: 12 Publishing Information: [Kew, England: Science and Technology Letters] Keywords: TIC Keywords: Agrostis stolonifera ; Cell culture ; Poa pratensis ; Suspension cultures ; Transgenes ; Transgenic turfgrasses Abstract/Contents: "To develop molecular strategies for gene containment in genetically modified (GM) turfgrass, we have studied the feasibility of using the FLP/FRT site-specific DNA recombination system from yeast for controlled genome modification in turfgrass. Suspension cell cultures of creeping bentgrass (Agrostis stolonifera L.) and Kentucky bluegrass (Poa pratensis ) were co-transformed with a FLP recombinase expression vector and a recombination-reporter test plasmid containing β-glucuronidase (gusA ) gene which was separated from the maize ubiquitin (ubi ) promoter by an FRT -flanked blocking DNA sequence to prevent its transcription. GUS activity was observed in co-transformed cells, in which molecular analyses indicated that FLP-mediated excision of the blocking sequence had brought into proximity the upstream promoter and the downstream reporter gene, resulting in GUS expression. Functional evaluation of the FLP/FRT system using transgenic creeping bentgrass stably expressing FLP recombinase confirmed the observation in suspension cell culture. Our results indicate that FLP/FRT system is a useful tool for genetic manipulation of turfgrass, pointing to the great potential of exploiting the system to develop molecular strategies for transgene containment in perennials." Language: English References: 47 Note: Pictures, color ASA/CSSA/SSSA Citation (Crop Science -Like - may be incomplete ): Biotechnol. lett. 28(22):p. 1793-1804.Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=146348 If there are problems with this record, send us feedback about record 146348 . Choices for finding the above item : DOI: 10.1007/s10529-006-9162-z Web URL(s): https://link.springer.com/article/10.1007%2Fs10529-006-9162-z b2322029~S1a Find from within TIC: Request through your local library's inter-library loan service (bring or send a copy of this TGIF record)
