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Publication Type:
| Refereed |
Author(s): | Boucaud, J.;
Bigot, J. |
Author Affiliation: | Laboratoire de Biochimie et Physiologie vegetale I.R.B.A., GS 79, Universite de Caen, F-14032 Caen Cedex, France. |
Title: | Changes in the Activities of Nitrogen Assimilation Enzymes of Lolium perenne L. During Regrowth After Cutting |
Source: | Plant and Soil. Vol. 114, No. 1, February 1989, p. 121-125. |
Publishing Information: | Dordrecht, Netherland: Martinus Nijhoff Publishers |
Related Web URL: | http://link.springer.com/article/10.1007/BF02203089 Last checked: 10/14/2015 Notes: Abstract only |
Keywords: | TIC Keywords: Lolium perenne; Nitrogen; Assimilation; Enzymes; Regrowth; Cutting; Nitrate reductase; Glutamine
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Abstract/Contents: | "The activities of key enxymes involved in N assimilation were investigated after defoliation of 6-week-old ryegrass plants grown in water culture conditions. In a first experiment, nitrate reductase, glutamine synthetase and glutamate dehydrogenase activities were measured in roots, stubble and leaves on the day of cutting and at 7-day intervals over the following 5-week period of regrowth. Ammonia assimilation enzymes showed little change whereas the nitrate reductase activity sharply decreased 2 weeks after clipping. In a second experiment, the nitrate reductase activity was measured at 2- or 3-day intervals 1 week before and 3 weeks after clipping. In vivo and in vitro assays both showed an increasing activity in leaves up to 8 days after cutting while root activity decreased. The opposite changes then occurred and both organs recovered their initial nitrate reductase activity levels after 12-14 days of regrowth. These fluctuations in nitrate reductase activity were considered to be related to the capacity for C assimilation and the nitrate availability." |
Language: | English |
References: | 28 |
Note: | Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Boucaud, J., and J. Bigot. 1989. Changes in the Activities of Nitrogen Assimilation Enzymes of Lolium perenne L. During Regrowth After Cutting. Plant Soil. 114(1):p. 121-125. |
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