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| Web URL(s): | http://archive.lib.msu.edu/tic/ressum/2009/9.pdf
Last checked: 01/23/2017
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Author(s): | Lakshman, Dilip K. |
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| Author Affiliation: | USDA-ARS |
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| Title: | Accurate identification and gene expression in relation to virulence of Rhizoctonia isolates infecting turfgrasses |
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| Section: | Integrated turfgrass management
Other records with the "Integrated turfgrass management" Section
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| Source: | 2009 Turfgrass and Environmental Research Summary. 2009, p. 9. |
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| Publishing Information: | Far Hills, NJ: United States Golf Association |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Disease identification; Gene expression; Rhizoctonia blight; Rhizoctonia cerealis; Rhizoctonia solani; Rhizoctonia zeae; Waitea circinata var. zeae
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| Abstract/Contents: | Presents a study conducted to determine the "molecular identification of Rhizoctonia solania isolates pathogenic to turfgrasses using Universally Primed-Polymerase Chain reaction (UP-PCR) and nucleic acid hybridization analysis." Describes the experiment and the methods used, explaining that "we collected a total of 448 Rhizoctonia samples from 5 locations in northern Virginia and Maryland during the summer of 2008 and 2009." |
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| Language: | English |
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| References: | 0 |
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| See Also: | Other Reports from this USGA research project: 2007-10-351 |
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| Note: | Pictures, b/w |
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| USGA Summary Points: | We have collected a total of 448 Rhizoctonia isolates from golf courses and lawns of Virginia and Maryland. Analyzing the rDNA-ITS sequence variations of 54 randomly sampled isolates, we found that majority (i.e., 61%) of the isolates belong to R. solani, while 22% belong to R. zeae/W. cercinata var circinata, and 17% belong to R. cerealis/Rhizoctonia-like fungi. Of the identified R. solani isolates in this study, majority belong to AG2-2IIIB and AG 1-1B. Genome fingerprinting of Rhizoctonia isolates using UP-PCR and AFLP techniques are in progress. Analyzing the two virulence-differentiated EST libraries, we have identified 532 and 495 unigenes of R. solani AG-4. Among several indentified genes are thsoe with possible roles as elicitors of disease, in cell wall degradation, avoidance of host and drug resistance and mating. |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Lakshman, D. K. 2009. Accurate identification and gene expression in relation to virulence of Rhizoctonia isolates infecting turfgrasses. Turfgrass Environ. Res. Summ. p. 9. |
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| Web URL(s):
http://archive.lib.msu.edu/tic/ressum/2009/9.pdf
Last checked: 01/23/2017
Requires: PDF Reader |
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MSU catalog number: b3609415 |
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