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DOI: | 10.1071/APP9850072 |
Web URL(s): | https://link.springer.com/article/10.1071%2FAPP9850072 Last checked: 10/05/2017 Access conditions: Item is within a limited-access website |
Publication Type:
| Refereed |
Author(s): | Hawkes, N. J.;
Harding, H. W. J. |
Author Affiliation: | Hawkes: Bayer Australia Ltd., Welland ; Harding: Forensic Science Center, Adelaide, South Australia |
Title: | Isoelectric focusing as an aid to the identification of Leptosphaeria narmeri, a cause of spring dead spot in turf |
Source: | Australasian Plant Pathology. Vol. 14, No. 4, December 1985, p. 72-76. |
Publishing Information: | Clayton, Vic. : Australian Plant Pathology Society |
# of Pages: | 5 |
Keywords: | TIC Keywords: Chemical analysis; Disease identification; Fungus infection; Ophiosphaerella narmari; Spring dead spot; pH
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Abstract/Contents: | " Isoelectric focusing ( IEF ) was investigated as an aide to identification of Leptosphaeria narmari, a fungus thought to be the main causal organsim in New South Wales of the turf disease commonly referred to as spring dead spot. Cultures of this fungus do not fruit on agar media so positive identification of cultures by taxonomic features of pseudothecia is impossible. Extracts obtained from fungal colonies grown in nutrient broth were separated by IEF, ph range 4.0-6.5. The protein banding patterns detected by staining with Serva Blue clearly differentiated Leptosphaeria narmari, L. maculans. Sixteen isolates from turf with spring dead spot from New South Wales and South Australia were also compared by IEF. Al isolates, except two, had a similar pattern of protein bands to an authentic isolate of L.narmari. |
Language: | English |
References: | 14 |
Note: | Tables Figures |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Hawkes, N. J., and H. W. J. Harding. 1985. Isoelectric focusing as an aid to the identification of Leptosphaeria narmeri, a cause of spring dead spot in turf. Australas. Plant Pathol. 14(4):p. 72-76. |
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| DOI: 10.1071/APP9850072 |
| Web URL(s): https://link.springer.com/article/10.1071%2FAPP9850072 Last checked: 10/05/2017 Access conditions: Item is within a limited-access website |
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