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DOI: | 10.1071/AP08077 |
Web URL(s): | https://link.springer.com/content/pdf/10.1071/AP08077.pdf Last checked: 10/05/2017 Requires: PDF Reader Access conditions: Item is within a limited-access website |
Publication Type:
| Refereed |
Author(s): | Dracatos, P. M.;
Dobrowolski, M. P.;
Lamb, J.;
Olle, R. S.;
Gendall, A. R.;
Cogan, N. O. I.;
Smith, K. F.;
Forster, J. W. |
Author Affiliation: | Dracatos, Dobrowolski, Lamb, Olle, Cogen, Smith, and Forster: Molecular Plant Breeding Cooperative Research Centre; Dracatos, Cogan, and Forster: Department of Primary Industries, Biosciences Research Division, Vicotiran AgriBiosciences Centre, La Trobe University Research and Development Park; Dracatos and Gendall: Department of Botany, Faculty of Science, Technology and Engineering, La Trobe University, Bundoora, Vic.; Dobrowolski, Lamb, Olle, and Smith: Department of Primary Industries, Biosciences Research Division, Hamilton Centre, Hamilton, Vic., Australia |
Title: | Development of genetically homogenised populations of the crown rust pathogen (Puccinia coronata f. sp. lolii) for disease trait dissection in perennial ryegrass (Lolium perenne L.) |
Source: | Australasian Plant Pathology. Vol. 38, No. 1, January 2009, p. 55-62. |
Publishing Information: | Clayton, Vic. : Australian Plant Pathology Society |
# of Pages: | 8 |
Related Web URL: | https://link.springer.com/article/10.1071/AP08077 Last checked: 10/05/2017 Notes: Abstract only |
Keywords: | TIC Keywords: Genetic analysis; Inoculum; Lolium perenne; Puccinia coronata var. coronata; Simple sequence repeats
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Abstract/Contents: | "Genetic analysis of host resistance to the crown rust pathogen of ryegrasses has traditionally involved the use of composite urediniospore collections, which may contain multiple genotypes and virulence races. Consequently, discrimination between major gene responses to alternative races and quantitative resistance has proved challenging for disease trait-dissection studies. A method of propagation on detached leaves has been used to derive genetically homogenised crown rust pathogen lines from bulked spore collections obtained from five distinct Australian locations. Spores from individual pustules were repeatedly reinoculated onto fresh leaves. Amplicon complexity was assessed at the conclusion of each cycle using crown rust pathogen expressed sequence tag (EST)-derived simple sequence repeat (SSR) primer pairs. The mean number of amplification products per isolate declined over successive rounds of reinfection. The number of rounds of sequential infection required for decay to stable levels was dependent on the diversity present within the bulked spore collection. Large-scale propagation of the putative homogenised populations developed in the present study may provide inoculum for infection studies to allow quantitative trait loci mapping of specific gene-for-gene responses. The methodology has significant applications for the pyramiding of resistance genes specific to diverse crown rust pathogen genotypes during cultivar development and for pathotyping of existing elite germplasm. This approach is also applicable to other Puccinia species." |
Language: | English |
References: | 27 |
Note: | Map Pictures, b/w Figures Tables Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Dracatos, P. M., M. P. Dobrowolski, J. Lamb, R. S. Olle, A. R. Gendall, N. O. I. Cogan, et al. 2009. Development of genetically homogenised populations of the crown rust pathogen (Puccinia coronata f. sp. lolii) for disease trait dissection in perennial ryegrass (Lolium perenne L.). Australas. Plant Pathol. 38(1):p. 55-62. |
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| DOI: 10.1071/AP08077 |
| Web URL(s): https://link.springer.com/content/pdf/10.1071/AP08077.pdf Last checked: 10/05/2017 Requires: PDF Reader Access conditions: Item is within a limited-access website |
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