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| Web URL(s): | https://scisoc.confex.com/scisoc/2020am/meetingapp.cgi/Paper/127018
Last checked: 09/07/2023 |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Tucker, Matthew;
Badial, Aline;
King, Jonas;
Tomaso-Peterson, Maria |
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| Author Affiliation: | Tucker: Student, Virginia Tech, Blacksburg, VA; Badial, King and Tomaso-Peterson: Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, Mississippi State, MS |
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| Title: | A multiplex qPCR approach for ectotrophic root-infecting fungal detection |
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| Section: | Molecular techniques, genetics and plant breeding oral (includes student competition)
Other records with the "Molecular techniques, genetics and plant breeding oral (includes student competition)" Section
C05 turfgrass science
Other records with the "C05 turfgrass science" Section
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| Meeting Info.: | San Antonio, Texas: November 9-13, 2020 |
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| Source: | ASA, CSSA, SSSA International Annual Meeting. November 2020, p. 127018. |
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| Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America] |
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| # of Pages: | 1 |
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| Abstract/Contents: | "This research focuses on a novel approach for ectotrophic root-infecting (ERI) fungal detection associated with bermudagrass decline (BD). Two summer core aerifications (July 2017 and July 2018) were used to collect root material from two golf greens at Mississippi State University Golf Institute. Greens were selected by BD symptomology based on superintendents suggestion of his healthiest (Green 2) and most symptomatic (Green 12) greens. Root material was collected from multiple areas of interest (AOI) within Green 2 (n=68) and Green 12 (n=66). Fungi of interest included the causal agent of BD, Gaeumannomyces graminis, as well as undescribed Gaeumannomyces sp., novel Candidacolonium cynodontis, and Magnaporthiopsis cynodontis. Two multiplex qPCR assays were used to identify the selected fungi, one determining presence and quantity of the two Gaeumannomyces spp. and ultradwarf bermudagrass (UDB) root, and the other determining presence and quantity of C. cynodontis, M. cynodontis, and UDB root. Multiple complexes of ERI fungi were identified in AOIs in both greens. Gaeumannomyces graminis, G. sp., C. cynodontis, and M. cynodontis were identified in 28, 78, 50, and 90% of AOIs, respectively, within Green 2 in 2017 as well as 0, 97, 51, and 100% in 2018. While inoculum densities for G. graminis, G. sp., and C. cynodontis were similar across years; inoculum density of M. cynodontis increased from 2017 to 2018 in Green 2. In Green 12, G. graminis, G. sp., C. cynodontis, and M. cynodontis were identified in 95, 20, 27, and 98% of AOIs, respectively, in 2017; as well as 48, 70, 51, and 97% in 2018. The inoculum density of G. graminis decreased from 2017 to 2018 in Green 12, whereas G. sp. and M. cynodontis increased across years and inoculum density of C. cynodontis was unchanged. Overall, colonization of roots by these select fungi occurred regardless of plant health." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Tucker, M., A. Badial, J. King, and M. Tomaso-Peterson. 2020. A multiplex qPCR approach for ectotrophic root-infecting fungal detection. Agron. Abr. p. 127018. |
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| Web URL(s):
https://scisoc.confex.com/scisoc/2020am/meetingapp.cgi/Paper/127018
Last checked: 09/07/2023 |
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