Full TGIF Record # 100756
Item 1 of 1
Web URL(s):http://turf.rutgers.edu/research/abstracts/symposium2005.pdf#page=51
    Last checked: 04/18/2008
    Requires: PDF Reader
Publication Type:
i
Report
Author(s):Rotter, David; Li, Huaijun Mike; Bonos, Stacy; Meyer, William; Belanger, Faith C.
Author Affiliation:Department of Plant Biology and Pathology, Rutgers University
Title:Identification of genes over-expressed in a colonial bentgrass x creeping bentgrass interspecific hybrid
Section:Poster presentations
Other records with the "Poster presentations" Section
Meeting Info.:Cook College, Rutgers, NJ: January 15-16, 1993
Source:Proceedings of the Second Annual Rutgers TurfgrassSymposium. 2005, p. 50.
Publishing Information:New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey
# of Pages:1
Keywords:TIC Keywords: Identification; Agrostis tenuis; Agrostis stolonifera; Genetic resistance; Hybrids; Dollar spot; Disease resistance; Gene expression; Gene mapping
Abstract/Contents:"Creeping bentgrass (Agrostis stolonifera L.) is highly susceptible to dollar spot disease caused by the fungus Sclerotinia homoeocarpa. Colonial bentgrass (A. capillaris L.) is a related species that has good dollar spot resistance. Interspecific hydrids between creeping and colonial bentgrass have been made and several hybrids exhibited resistance to dollar spot. The molecular basis for this resistance can be investigated by using suppression subtractive hybribization to detect low copy number transcripts that are unique or over-expressed in either the resistant hybrids or the creeping bentgrass parent following fungal inoculation. RNA was isolated from field samples when creeping lines exhibited dollar spot symptoms. Two subtraction libraries were created from creeping and colonial bentgrass cDNAs, one is enriched for genes in the hybrid relative to the creeping parent, and the other is enriched for genes in the creeping parent relative to the hybrid. We are now carrying out RNA blot analysis on the field samples to evaluate relative expression levels of the differentially expressed genes. A beta-glucosidase and 14-3-3 protein were dramatically over-expressed in the hybrid relative to its creeping parent in greenhouse grown plants and both were upregulated in field samples. The resistant hybrid was crossed to a creeping plant to generate a backcross population that was evaluated for dollar spot resistance in 2004. This population will be used for mapping the colonial bentgrass genome and determine if any of the differentially expressed genes can be correlated with the resistance trait."
Language:English
References:0
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Rotter, D., H. M. Li, S. Bonos, W. Meyer, and F. C. Belanger. 2005. Identification of genes over-expressed in a colonial bentgrass x creeping bentgrass interspecific hybrid. Proc. Annu. Rutgers Turfgrass Symp. p. 50.
Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=100756
If there are problems with this record, send us feedback about record 100756.
Choices for finding the above item:
Web URL(s):
http://turf.rutgers.edu/research/abstracts/symposium2005.pdf#page=51
    Last checked: 04/18/2008
    Requires: PDF Reader
Find Item @ MSU
MSU catalog number: SB 433 .R88
Find from within TIC:
   Digitally in TIC by file name: rutsy2005
Request through your local library's inter-library loan service (bring or send a copy of this TGIF record)