Full TGIF Record # 100773
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Web URL(s):http://turf.rutgers.edu/research/abstracts/symposium2005.pdf#page=55
    Last checked: 11/26/2007
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Publication Type:
i
Report
Content Type:Abstract or Summary only
Author(s):Zhang, Eddie; Hu, Suli; Xing, Jinpeng; Gianfagna, Thomas
Author Affiliation:Department of Plant Biology and Pathology, Rutgers University
Title:In vitro selection for salt tolerant turfgrass plants
Section:Poster presentations
Other records with the "Poster presentations" Section
Meeting Info.:Cook College, Rutgers, NJ: January 13-14, 2005
Source:Proceedings of the Fourteenth Annual Rutgers Turfgrass Symposium. 2005, p. 54.
Publishing Information:New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey
# of Pages:1
Keywords:TIC Keywords: Choice of species; In vitro; Breeding improvement; Salt tolerance; Salinity stress; Calli
Abstract/Contents:"Turfgrasses are being subjected to rising amounts of salinity stress, resulting from increased salinization of agricultural areas and use of non-potable water sources as a method of turfgrass irrigation. The objective of this study was to determine the feasibility of an in vitro method for selecting salt tolerant turfgrasses. The information will provide a meteric for determining the optimal salinity content in non-potable water sources, and the feasibility of irrigating turfgrasses in a high salt environment. Seeds of creeping bentgrass (cv. Penncross) were sterilized and calli were generated in MS medium containing varying solutions of NaCl (0%, 0.5%, 0.75%, 1.0%, 1.25%, 1.5%). The growth rates of calli were evaluated using surface area measurements taken with a digital image analysis program. After 28 days of culture, surface area of the control calli increased 47%. Similarly, the calli exposed to 0.5% NaCl concentration showed a surface area increase of 48%. However, calli in 0.75% and 1.0% NaCl increased only by 35% and 42% in size respectively. At a high level of salinity, surface area increases were only 20% at 1.25% NaCl and 13% for 1.5% NaCl. Despite showing a significant decrease in growth rate a number of calli in the 1.25% - 1.5% salinity range still showed characteristics of growth after 28 days. Surviving calli from the 1.25% range were trasferred to a 2.0% NaCl concentration medium, and subsequently regenerated into plants on 0% NaCl media for further tests of salinity tolerance. Calli cultured at 1.25% NaCl maintained higher survival rate when transferred to 2.0% NaCl, while those calli grown previously at 0% NaCl lost viability quickly when transferred to 2.0% NaCl medium. These results suggest that the callus selected through increasing concentration of salt would likely generate turfgrass plants that have greater tolerance to high salinity."
Language:English
References:0
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zhang, E., S. Hu, J. Xing, and T. Gianfagna. 2005. In vitro selection for salt tolerant turfgrass plants. Proc. Annu. Rutgers Turfgrass Symp. p. 54.
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Web URL(s):
http://turf.rutgers.edu/research/abstracts/symposium2005.pdf#page=55
    Last checked: 11/26/2007
    Requires: PDF Reader
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MSU catalog number: SB 433 .R88
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