| |
Web URL(s): | http://turf.rutgers.edu/research/abstracts/symposium2005.pdf#page=55 Last checked: 11/26/2007 Requires: PDF Reader |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Zhang, Eddie;
Hu, Suli;
Xing, Jinpeng;
Gianfagna, Thomas |
Author Affiliation: | Department of Plant Biology and Pathology, Rutgers University |
Title: | In vitro selection for salt tolerant turfgrass plants |
Section: | Poster presentations Other records with the "Poster presentations" Section
|
Meeting Info.: | Cook College, Rutgers, NJ: January 13-14, 2005 |
Source: | Proceedings of the Fourteenth Annual Rutgers Turfgrass Symposium. 2005, p. 54. |
Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
# of Pages: | 1 |
Keywords: | TIC Keywords: Choice of species; In vitro; Breeding improvement; Salt tolerance; Salinity stress; Calli
|
Abstract/Contents: | "Turfgrasses are being subjected to rising amounts of salinity stress, resulting from increased salinization of agricultural areas and use of non-potable water sources as a method of turfgrass irrigation. The objective of this study was to determine the feasibility of an in vitro method for selecting salt tolerant turfgrasses. The information will provide a meteric for determining the optimal salinity content in non-potable water sources, and the feasibility of irrigating turfgrasses in a high salt environment. Seeds of creeping bentgrass (cv. Penncross) were sterilized and calli were generated in MS medium containing varying solutions of NaCl (0%, 0.5%, 0.75%, 1.0%, 1.25%, 1.5%). The growth rates of calli were evaluated using surface area measurements taken with a digital image analysis program. After 28 days of culture, surface area of the control calli increased 47%. Similarly, the calli exposed to 0.5% NaCl concentration showed a surface area increase of 48%. However, calli in 0.75% and 1.0% NaCl increased only by 35% and 42% in size respectively. At a high level of salinity, surface area increases were only 20% at 1.25% NaCl and 13% for 1.5% NaCl. Despite showing a significant decrease in growth rate a number of calli in the 1.25% - 1.5% salinity range still showed characteristics of growth after 28 days. Surviving calli from the 1.25% range were trasferred to a 2.0% NaCl concentration medium, and subsequently regenerated into plants on 0% NaCl media for further tests of salinity tolerance. Calli cultured at 1.25% NaCl maintained higher survival rate when transferred to 2.0% NaCl, while those calli grown previously at 0% NaCl lost viability quickly when transferred to 2.0% NaCl medium. These results suggest that the callus selected through increasing concentration of salt would likely generate turfgrass plants that have greater tolerance to high salinity." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Zhang, E., S. Hu, J. Xing, and T. Gianfagna. 2005. In vitro selection for salt tolerant turfgrass plants. Proc. Annu. Rutgers Turfgrass Symp. p. 54. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=100773 |
| If there are problems with this record, send us feedback about record 100773. |
| Choices for finding the above item: |
| Web URL(s): http://turf.rutgers.edu/research/abstracts/symposium2005.pdf#page=55 Last checked: 11/26/2007 Requires: PDF Reader |
| MSU catalog number: SB 433 .R88 |
| Find from within TIC: Digitally in TIC by file name: rutsy2005 |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |