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| DOI: | 10.1007/s00122-033-1428-4 |
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| Web URL(s): | https://link.springer.com/content/pdf/10.1007%2Fs00122-003-1428-4.pdf
Last checked: 10/03/2017
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Budak, H.;
Shearman, R. C.;
Parmaksiz, I.;
Gaussoin, R. E.;
Riordan, T. P.;
Dweikat, I. |
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| Author Affiliation: | Department of Agronomy and Horticulture, University of Nebraska, Lincoln, Nebraska |
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| Title: | Molecular characterization of buffalograss germplasm using sequence-related amplified polymorphism markers |
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| Source: | Theoretical and Applied Genetics. Vol. 108, No. 2, January 2004, p. 328-334. |
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| Publishing Information: | Berlin: Springer |
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| # of Pages: | 7 |
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| Related Web URL: | https://link.springer.com/article/10.1007%2Fs00122-003-1428-4
Last checked: 10/03/2017
Notes: Abstract only |
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| Keywords: | TIC Keywords: Bouteloua dactyloides; Simple sequence repeats; Genetic markers; Genetic diversity; Ploidy
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| Abstract/Contents: | "Buffalograss [Buchloe dactyloids (Nutt.) Englem] germplasm has a broad resource of genetic diversity that can be used for turfgass, forage, and conservation. Buffalograss is the only native grass that is presently used as turfgrass in the Great Plains region of North America. Its low growth habit, drought tolerance and reduced requirement for fertilizer and pesticides contribute to interest in its use. The objectives of this study were to use sequence-related amplified polymorphism (SRAP) markers in the evaluation of genetic diversity and phenetic relationships in a diverse collection of 53 buffalograss germplasms, and to identify buffalograss ploidy levels using flow cytometry. Based on their DNA conents, buffalograss genotypes were grouped into four sets, corresponding to their ploidy levels. Thirty-four SRAP primer combinations were used. This is the first report of the detection of differentiating diploid, tetraploid, pentaploid and hexaploid buffalograss genotypes, representing diverse locations of origin, using SRAP markers. Cluster analysis by the unweighted pair-group method with arithmetic averages based on genetic similarity matricies indicated that there were eight clusters. The coefficients of genetic distance among the genotypes, ranged from 0.33 up to 0.99 and averaged D=0.66. The genetic diversity estimate, He, averaged 0.35. These results demonstrated that genotypes with potential traits for turfgrass improvement could readily be distinguished, based on SRAP. The use of PCR-based technologies such as SRAP is an effective tool for estimating genetic diversity, identifying unique genotypes as new sources of alleles for enhancing turf characteristics, and for analyzing the evolutionary and historical development of cultivars at the genomic level in a buffalograss breeding program." |
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| Language: | English |
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| References: | 20 |
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| Note: | Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Shearman, R. C., I. Parmaksiz, R. E. Gaussoin, I. Dweikat, and H. Budak. 2004. Molecular characterization of buffalograss germplasm using sequence-related amplified polymorphism markers. Theor. Appl. Genet. 108(2):p. 328-334. |
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| DOI: 10.1007/s00122-033-1428-4 |
| Web URL(s):
https://link.springer.com/content/pdf/10.1007%2Fs00122-003-1428-4.pdf
Last checked: 10/03/2017
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
| 
MSU catalog number: QH 426 .T47 |
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