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| DOI: | 10.1007/s001220051661 |
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| Web URL(s): | https://link.springer.com/content/pdf/10.1007%2Fs001220051661.pdf
Last checked: 10/03/2017
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Jones, E. S.;
Dupal, M. P.;
Kölliker, R.;
Drayton, M. C.;
Forster, J. W. |
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| Author Affiliation: | Jones, Dupal, Drayton and Forster: Plant Biotechnology Centre, Agriculture Victoria, La Trobe University, Bundoora, Victoria and CRC, Molecular Breeding, Australia; Kölliker: Swiss Federal Research Station, Agroecology and Agriculture, Zürich, Switzerland |
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| Title: | Development and characterisation [characterization] of simple sequence repeat (SSR) markers for perennial ryegrass (Lolium perenne L.) |
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| Source: | Theoretical and Applied Genetics. Vol. 102, No. 2/3, February 2001, p. 405-415. |
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| Publishing Information: | Berlin: Springer |
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| # of Pages: | 11 |
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| Related Web URL: | https://link.springer.com/article/10.1007%2Fs001220051661
Last checked: 10/03/2017
Notes: Abstract only |
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| Keywords: | TIC Keywords: Simple sequence repeats; Lolium perenne; Genetic markers; Loci; DNA
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| Abstract/Contents: | "Enrichment methods were optimised [optimized] in order to isolate large numbers of simple sequence repeat (SSR) markers for perennial ryegrass (Lolium perenne L.), with the aim of developing a comprehensive set of loci for trait mapping and cultivar identification. Two libraries were constructed showing greater than 50% enrichment for a variety of SSR-motif types. Sequence characterisation [characterization] of 1853 clones identified 859 SSR-containing clones, of which 718 were unique. Truncation of flanking sequences limited potential primer design to 366 clones. One-hundred selected SSR primer pairs were evaluated for amplification and genetic polymorphism across a panel of diverse genotypes. The efficiency of amplification was 81%. A relatively high level of SSR polymorphism was detected (67%), with a range of 2-7 alleles per locus. Mendelian segregation of alleles detected by selected SSR-locus primer pairs was demonstrated in the F1 progeny of a pair cross. Cross-species amplification was detected in a number of related pasture and turfgrass species, with high levels of transfer to other Lolium species and members of the related genus Festuca. The identity of putative SSR ortholoci in these related species was confirmed by DNA sequence analysis. These loci constitute a valuable resource of ideal markers for the molecular breeding of ryegrasses and fescues." |
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| Language: | English |
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| References: | 60 |
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| Note: | Pictures, b/w
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Jones, E. S., M. P. Dupal, R. Kölliker, M. C. Drayton, and J. W. Forster. 2001. Development and characterisation [characterization] of simple sequence repeat (SSR) markers for perennial ryegrass (Lolium perenne L.). Theor. Appl. Genet. 102(2/3):p. 405-415. |
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| DOI: 10.1007/s001220051661 |
| Web URL(s):
https://link.springer.com/content/pdf/10.1007%2Fs001220051661.pdf
Last checked: 10/03/2017
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
| 
MSU catalog number: QH 426 .T47 |
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