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Web URL(s): | https://dl.sciencesocieties.org/publications/cs/articles/45/3/1069 Last checked: 11/16/2016 Access conditions: Item is within a limited-access website https://dl.sciencesocieties.org/publications/cs/pdfs/45/3/1069 Last checked: 11/16/2016 Requires: PDF Reader Access conditions: Item is within a limited-access website |
Publication Type:
| Refereed |
Author(s): | Jain, Mukesh;
Chengalrayan, Kudithipudi;
Gallo-Meagher, Maria;
Mislevy, Paul |
Author Affiliation: | Jain and Chengalrayan: Department of Agronomy, University of Florida, Gainesville, Florida; Gallo-Meagher: Department of Agronomy, Plant Molecular and Cellular Biology Program, and Genetics Institute, University of Florida, Gainesville, Florida; Mislevy: Range Cattle Research and Education Center, University of Florida, Ona, Florida |
Title: | Embryogenic callus induction and regeneration in a pentaploid hybrid bermudagrass cv. Tifton 85 |
Section: | Notes Other records with the "Notes" Section
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Source: | Crop Science. Vol. 45, No. 3, May/June 2005, p. 1069-1072. |
Publishing Information: | Madison, WI: Crop Science Society of America |
# of Pages: | 4 |
Related Web URL: | https://dl.sciencesocieties.org/publications/cs/abstracts/45/3/1069 Last checked: 11/16/2016 Notes: Abstract only |
Keywords: | TIC Keywords: Somatic embryogenesis; Regeneration; Hybrid bermudagrasses; Calli; Injuries by insects; Pest control; Inflorescences; Morphogenesis; Sucrose; 2,4-D; Cytokinins; Casein; Plant protein hydrolysates; Phenotypic plasticity; Cynodon dactylon; Cynodon transvaalensis
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Cultivar Names: | Tifton 85 |
Abstract/Contents: | "Hybrid bermudagrass [Cynodon dactylon (L.) Pers. x C. transvaalensis Burtt Davy] cv. Tifton 85 is a major forage grass in the southeastern USA that suffers severe yield losses because of insect damage. A facile, cultivar-specific regeneration protocol is required to pursue genetic improvement of Tifton 85 for incorporating insect resistance into this valuable forage crop. An efficient regeneration system that uses somatic embryogenesis from immature inflorescences of Tifton 85 has been established. Young, immature inflorescence explants proved to be an excellent source for obtaining embryogenic callus, as opposed to apical meristems and nodal segments. Embryogenic callus with demonstrable morphogenetic competence was obtained on MS basal medium containing 30 g L-1 sucrose, 4 mg L-1; 2,4-dichlorophenoxyacetic acid (2,4-D), 0.01 mg L-1 6-benzylaminopurine (BAP) and 200 mg L-1 casein hydrolysate. The regenerants were rooted on hormone free MS basal medium supplemented with 30 g L-1 sucrose, successfully established in soil under greenhouse conditions, and did not show any phenotypic differences compared with wild-type Tifton 85 plants." |
Language: | English |
References: | 15 |
See Also: | Other items relating to: Biostimulants
Other items relating to: 2, 4 - D in Turf |
Note: | Pictures, b/w Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Jain, M., K. Chengalrayan, M. Gallo-Meagher, and P. Mislevy. 2005. Embryogenic callus induction and regeneration in a pentaploid hybrid bermudagrass cv. Tifton 85. Crop Sci. 45(3):p. 1069-1072. |
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| Web URL(s): https://dl.sciencesocieties.org/publications/cs/articles/45/3/1069 Last checked: 11/16/2016 Access conditions: Item is within a limited-access website https://dl.sciencesocieties.org/publications/cs/pdfs/45/3/1069 Last checked: 11/16/2016 Requires: PDF Reader Access conditions: Item is within a limited-access website |
| MSU catalog number: SB 183 .C7 |
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