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Web URL(s):https://archive.lib.msu.edu/tic/its/articles/2005jou490.pdf
    Last checked: 08/09/2010
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Publication Type:
i
Refereed
Author(s):Barker, Reed E.; Warnke, Scott E.; Brown, Rebecca Nelson
Author Affiliation:Barker: USDA-ARS National Forage Seed Production Research Center, Corvalis, Oregon; Warnke: National Arboretum, USDA-ARS, Washington, DC; Brown: Oregon State University, Corvalis, Oregon
Title:Genetic variability among hybrid ryegrass seed lots and cultivars detected by RAPD analysis
Section:Genetics, breeding & variety evaluation
Other records with the "Genetics, breeding & variety evaluation" Section
Meeting Info.:Llandudno, Wales, UK: July 10-15 2005
Source:International Turfgrass Society Research Journal. Vol. 10, No. Part 1, 2005, p. 490-494.
Publishing Information:Aberystywth, Ceredigion, UK: International Turfgrass Society
# of Pages:5
Keywords:TIC Keywords: Genetic variability; Lolium x hybridum; Lolium multiflorum; Random amplified polymorphic DNA markers; Seed purity; Lolium perenne; Seed quality; DNA amplification
Abstract/Contents:"High quality turf can only be obtained using highly pure seed. Seed purity is a significant challenge in perennial ryegrass (Lolium perenne L.) seed production. Annual (Italian) ryegrass (L. multiforum Lam.) is widespread in Oregon as both a crop and a weed. Hybrid ryegrass (Lolium X hybridum Hausskn.) is a hybrid between the two species and is used to overseed sports turfs in the southern USA because it establishes quickly and is expected to readily die in late spring/early summer heat. Fifteen greenhouse grown plants from each of three experimental lines and two cultivars were examined by RAPD analyses. Four seed lots for each of two experimental lines were included. Only clearly distinct polymorphic bands were used to construct DNA profiles. Genetic distances between individual pairs were computed and distance matrices analyzed using AMOVA. Relationships were visualized by 3-dimensional graphs of principle components. Over 93% of the genetic variation was within accessions, leaving only 7% to distinguish accessions using RAPDs. The between accession variation was enough, however, to show that eight seed lots from the two experimental lines were indistinguishable. The other experimental line and the two cultivars were clearly distinct from each other and from the two lines that were related. DNA profiling is a valuable tool to distinguish accessions of hybrid ryegrass and to provide initial information on genetic relatedness among plant types."
Language:English
References:18
Note:Figures
Tables
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Barker, R. E., S. E. Warnke, and R. N. Brown. 2005. Genetic variability among hybrid ryegrass seed lots and cultivars detected by RAPD analysis. Int. Turfgrass Soc. Res. J. 10(Part 1):p. 490-494.
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https://archive.lib.msu.edu/tic/its/articles/2005jou490.pdf
    Last checked: 08/09/2010
    Requires: PDF Reader
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MSU catalog number: SB 433 .I52 v. 10
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