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| Web URL(s): | https://archive.lib.msu.edu/tic/its/articles/2005jou550.pdf
Last checked: 08/09/2010
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| Publication Type:
| Refereed |
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| Author(s): | Fei, S.;
Riordan, T.;
Bishnoi, U.;
Clemente, T. |
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| Author Affiliation: | Fei: Department of Horticulture, Iowa State University, Ames, Iowa; Riordan, Bishnoi, and Clemente: Department of Agronomy and Horticulture, University of Nebraska, Lincoln, Nebraska |
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| Title: | Regeneration of transgenic buffalograss (Buchloe dactyloides) with glyphosate resistance using particle bombardment |
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| Section: | Genetics, breeding & variety evaluation
Other records with the "Genetics, breeding & variety evaluation" Section
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| Meeting Info.: | Llandudno, Wales, UK: July 10-15 2005 |
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| Source: | International Turfgrass Society Research Journal. Vol. 10, No. Part 1, 2005, p. 550-554. |
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| Publishing Information: | Aberystywth, Ceredigion, UK: International Turfgrass Society |
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| # of Pages: | 5 |
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| Keywords: | TIC Keywords: Regeneration; Bouteloua dactyloides; Glyphosate; Particle bombardment; Growth rate; Transgenic turfgrasses; Herbicide resistance; Weed control; Genetic transformation
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| Abstract/Contents: | "The use of buffalograss as a low maintenance turfgrass has increased in the past decade because of its excellent drought tolerance and improved turf quality. One of the challenges of growing buffalograss, however, is its slow growth rate which may cause weed infestation during establishment. Developing buffalograss with Roundup® tolerance may provide an effective strategy to control grassy weeds, many of which respond to herbicides similarly as buffalograss. Here we report the establishment of a transformation protocol using particle bombardment for transferring the 5-enolpyruvylshikimate phosphate synthase gene (cp4 epsps) into embryogenic callus and subsequently recovering transgenic plants for a turf-type vegetative buffalograss cultivar '91-118'. Southern blot and Trait RUR Lateral Flow Test confirmed the successful integration of the cp4 epsps gene into the buffalograss genome and the production of the CP4 EPSPS enzyme in transgenic buffalograss plants. A delay for selection increased callus transformation efficiency. A delay of either 7 or 28 days for selection yielded 1.7 ± 0.5% and 2.0 ± 0.3% of glyphosate resistant calluses, respectively while a delay of 3 days yielded only 0.4 ± 0.4% of glyphosate resistant callus. The selection level also had an effect on the transformation efficiency; selection medium containing 1 mM glyphosate yielded an average of 8.0 ± 3.1% of glyphosate resistant calluses, while the selection medium containing 2 mM glyphosate resulted in an average of 2.5 ± 0.8% resistant calluses" |
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| Language: | English |
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| References: | 19 |
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| Note: | Pictures, b/w
Figures |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Fei, S., T. Riordan, U. Bishnoi, and T. Clemente. 2005. Regeneration of transgenic buffalograss (Buchloe dactyloides) with glyphosate resistance using particle bombardment. Int. Turfgrass Soc. Res. J. 10(Part 1):p. 550-554. |
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| Web URL(s):
https://archive.lib.msu.edu/tic/its/articles/2005jou550.pdf
Last checked: 08/09/2010
Requires: PDF Reader |
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MSU catalog number: SB 433 .I52 v. 10 |
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