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| Publication Type:
| Refereed |
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| Author(s): | Hughes, Sheena J.;
Thorogood, Danny |
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| Author Affiliation: | Department of Plant Genetics and Breeding, Institute of Grassland and Environmental Research, Aberystwyth, Ceredigion, United Kingdom |
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| Title: | Distinguishing Poa supina, and P. annua by species specific isozyme alleles and flow cytometry |
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| Section: | Genetics, breeding & variety evaluations
Other records with the "Genetics, breeding & variety evaluations" Section
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| Meeting Info.: | Llandudno, Wales, UK: July 10-15 2005 |
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| Source: | International Turfgrass Society Research Journal. Vol. 10, No. Part 1, 2005, p. 654-660. |
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| Publishing Information: | Aberystywth, Ceredigion, UK: International Turfgrass Society |
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| # of Pages: | 7 |
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| Keywords: | TIC Keywords: Poa supina; Poa annua; Isoenzymes; Laser flow cytometry; Seed purity; Alleles; Identification keys; Contamination; Seed quality; Testing
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| Abstract/Contents: | "Poa supina is a grass used commercially as a turfgrass on sportsgrounds, it has an aggressive nature and rapid growth rate which contributes to its wear tolerance. P. supina is pereferable to the closely related annual meadow grass, P. annua, because it does not produce flowering heads all-year-round which can affect the playing quality and aesthetics of the turf surface. It is important to be able to distinguish the two species in order to determine the levels of P. annua contamination within commercial stocks of P. supina which might adversely affect the quality of the seed mix. Methods for distinguishing Poa annua and P. supina on the basis of vernalisation requirement, isozymes analysis and ploidy are described and compared. We have used electrophoretic assays of the PGI isozyme and flow cytometry to asses the levels of contamination in a number of commercial stocks of P. supina. Considerable variation for the number of contaminant P. annua plants (0-24%) were found in commercial P. supina stocks. One accession classified as P. supina, originating from India and stored by the Genetic Resources Information Network, was found to be completely misclassified and was most probably a collection of P. annua. Contaminant individuals could be reliably detected in bulked samples prepared from the leaves of as many as five plants using isozymes. Using flow cytometry a contaminant individual can be detected in a bulked sample derived from fifteen different plants. Our overall assessment is that flow cytometry is a quicker, more cost effective method of screening plants than using isozyme electrophoresis." |
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| Language: | English |
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| References: | 7 |
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| Note: | Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Hughes, S. J., and D. Thorogood. 2005. Distinguishing Poa supina, and P. annua by species specific isozyme alleles and flow cytometry. Int. Turfgrass Soc. Res. J. 10(Part 1):p. 654-660. |
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