Full TGIF Record # 106263
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Author(s):Grønnerød, Siri; Fjellheim, Siri; Humphreys, Michael W.; Østrem, Liv; Canter, Peter H.; Grieg, Zanina; Jørgensen, Øyvind; Larsen, Arild; Rognli, Odd Arne
Author Affiliation:Grønnerød, Fjellheim, Grieg, Jørgensen & Rognli: Department of Chemistry and Biotechnology, Agricultural University of Norway,Aas, Norway; Humphreys & Canter: Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Wales, United Kingdom; Østrem: Norwegian Crop Research Institute, Furenest, Norway; Larsen: Norwegian Crop Research Institute, V^Doaønes Research Station, Bodø, Norway
Title:Application of AFLP and GISH techniques for indentification of Festuca chromosome segments conferring winter hardiness in a Lolium perenne x Festuca pratensis population
Section:Tolerance to abiotic stress
Other records with the "Tolerance to abiotic stress" Section
Meeting Info.:Third International Symposium, Molecular Breeding of Forage and Turf, Dallas, Texas and Ardmore, Oklahoma, May 18-22, 2003
Source:Molecular Breeding of Forage and Turf: Proceedings of the 3rd International Symposium, Molecular Breeding of Forage and Turf. 2004, p. 81-86.
# of Pages:6
Publishing Information:Dordrecht: Kluwer Academic Publishers
Series:Developments in Plant Breeding Volume 11
Keywords:TIC Keywords: Festuca; Chromosome maps; Hybridization; Chromosomes; Cold resistance; Lolium perenne; Festuca pratensis; Amplification fragment length polymorphisms; Introgression; Breeding program
Cultivar Names:Prior
Abstract/Contents:"Diploid BC1, BC2, and BC3 introgression lines derived from the amphiploid Lolium perenne x Festuca pratensis cv. Prior were scored for freezing tolerance and analysed using AFLP markers and GISH in order to associate molecular markers to introgressed Festuca segments. The freezing tests showed highly significant differences between clones of the introgression lines. Two introgression lines were more frost tolerant than the diploid Lolium-control cultivars. Thirteen AFLP-primer combinations were tested. It was not possible to identify single markers that were present in the most freezing tolerant plants, and absent in the most susceptible ones. The GISH analyses of 20 diploid PriorBC1-plants with Lolium-morphology showed that 3 genotypes had no visible Festuca-segments, while the others had 1-5 segments of different sizes. Analyses of a PriorBC3 population showed that introgression in the satellite region of chromosome 3 seem to confer enhanced frost tolerance to Lolium. Winter hardiness and freezing tolerance are quantitative traits governed by genes on different chromosomes, and combinations of AFLP markers tagging different chromosomal regions may be used together o select superior genotypes during introgression. AFLP markers most frequently present in plants with the highest freezing tolerance, and absent in plants with winter inferior freezing tolerance, were therefore chosen and associated with hardiness and with the presence of Festuca introgressions. These markers may be useful in marker-assisted breeding programmes."
ISBN:1-4020-1867-3
Language:English
References:9
Note:Tables
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
2004. Application of AFLP and GISH techniques for indentification of Festuca chromosome segments conferring winter hardiness in a Lolium perenne x Festuca pratensis population. Molecular Breeding of Forage and Turf: Proceedings of the 3rd International Symposium, Molecular Breeding of Forage and Turf. p. 81-86.
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