Full TGIF Record # 106265
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Publication Type:
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Report
Author(s):Zhang, Yan; Zwonitzer, John; Chekhovskiy, Konstantin; May, Gregory D.; Rouf Mian, M. A.
Author Affiliation:Zhang, Zwonitzer, Chekhovskiy & Rouf Mian: Forage Improvement Division; and May: Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma
Title:A functional genomics approach for identification of heat tolerance genes in tall fescue
Section:Tolerance to abiotic stress
Other records with the "Tolerance to abiotic stress" Section
Meeting Info.:Third International Symposium, Molecular Breeding of Forage and Turf, Dallas, Texas and Ardmore, Oklahoma, May 18-22, 2003
Source:Molecular Breeding of Forage and Turf: Proceedings of the 3rd International Symposium, Molecular Breeding of Forage and Turf. 2004, p. 87-96.
# of Pages:10
Publishing Information:Dordrecht: Kluwer Academic Publishers
Series:Developments in Plant Breeding Volume 11
Keywords:TIC Keywords: Genomes; Heat resistance; Festuca arundinacea; Heat stress; cDNA library; Gene expression; Genotypes; DNA; Hybridization
Cultivar Names:Kentucky 31
Abstract/Contents:"Tall fescue (Festuca arundinacea Schreb.) is a major cool season forage and turf grass species around the world. Heat stress is one of the limiting factors for forage production and turf management of this grass species. This research uses functional genomics approaches for discovering the molecular basis of heat tolerance in tall fescue genotypes. To identify genes expressed during heat stress, a heat stressed shoot cDNA library was constructed from popular tall fescue cultivar Kentucky 31 with shoot tissues collected at five time points (12-78 hrs) after exposure to high heat (42-44◦C) in a growth chamber. High-throughput sequencing of the cDNA clones is underway for generating 5,000 ESTs. The ESTs related to heat-stress will be identified from this cDNA library. Heat-tolerance related genes are cloned by using suppression subtractive hybridization (SSH) between heat tolerant and heat sensitive cultivars. Heat tolerance-related genes isolated by SSH will be combined with genes cloned from shoot cDNA library to generate a cDNA microarray to study the expression profiles of tall fescue cultivars with different response to the heat stress. Heat tolerance genes identified from this project will be useful for development of tall fescue cultivars adapted to high heat environments."
ISBN:1-4020-1867-3
Language:English
References:12
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
2004. A functional genomics approach for identification of heat tolerance genes in tall fescue. Molecular Breeding of Forage and Turf: Proceedings of the 3rd International Symposium, Molecular Breeding of Forage and Turf. p. 87-96.
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