Full TGIF Record # 106271
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Publication Type:
i
Report
Author(s):Felitti, S.; Shields, K.; Ramsperger, M.; Tian, P.; Webster, T.; Ong, B.; Sawbridge, T.; Spangenberg, G.
Author Affiliation:Felitti, Shields, Ramsperger & Tian: Plant Biotechnology Centre, Department of Primary Industries, La Trobe University, Bundoora, Victoria, Australia and CRC for Molecular Plant Breeding; Webster, Ong, Sawbridge & Spangenberg: Victorian Microarray Technology Consortium; Sawbridge and Spangenberg: Victorian Bioinformatic Consortium, Australia
Title:Gene discovery and microarray-based transcriptome analysis in grass endophytes
Section:Genomics of plant-symbiont relations
Other records with the "Genomics of plant-symbiont relations" Section
Meeting Info.:Third International Symposium, Molecular Breeding of Forage and Turf, Dallas, Texas and Ardmore, Oklahoma, May 18-22, 2003
Source:Molecular Breeding of Forage and Turf: Proceedings of the 3rd International Symposium, Molecular Breeding of Forage and Turf. 2004, p. 145-153.
# of Pages:9
Publishing Information:Dordrecht: Kluwer Academic Publishers
Series:Developments in Plant Breeding Volume 11
Keywords:TIC Keywords: Genes; Epichloe; Endophytes; Symbiosis; Genomes; Gene expression; Analysis; In vitro; Hybridization
Abstract/Contents:"A genomic resource of 9411 expressed sequence tags (ESTs) representing 5038 genes has been established for Neotyphodium and Epichlo√ę endophytes. A cDNA spotted microarray interrogating 4195 Neotyphodium and 920 Epichlo√ę genes (EndoChip) has been generated. It provides a tool for high-throughput transcriptional profiling of endophytes, genome-specific gene expression analysis and expression profiling of novel endophyte genes, as well as transciptome analysis in the grass-endophyte association. Microarray-based gene expression analysis using RNA from in vitro grown N. coenophialum and N. lolii endophytes demonstrated its utility in comparative transcriptome analysis of both endophyte species. Additional proof of concept was obtained in using the EndoChip to analyse genome wide-gene expression in endophytes grown in vitro and in planta and for gene expression profiling of novel genes based on coordinated expression with genes of known cellular functions. Northern hybridisation analysis allowed for validation of microarray-based gene expression data. In combination with established unigene microarrays for the host grasses it provides a tool for the dissection of the grass-endophyte association at the transcriptome level."
ISBN:1-4020-1867-3
Language:English
References:1
Note:Figures
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
2004. Gene discovery and microarray-based transcriptome analysis in grass endophytes. Molecular Breeding of Forage and Turf: Proceedings of the 3rd International Symposium, Molecular Breeding of Forage and Turf. p. 145-153.
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