Full TGIF Record # 106296
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Publication Type:
i
Report
Author(s):Luo, Hong; Hu, Qian; Nelson, Kimberly; Longo, Chip; Kausch, Albert P.
Author Affiliation:HybriGene Inc., West Kingston, Rhode Island
Title:Controlling transgene escape in genetically modified grasses
Section:Development and application of molecular technologies in forage and turf improvement
Other records with the "Development and application of molecular technologies in forage and turf improvement" Section
Meeting Info.:Third International Symposium, Molecular Breeding of Forage and Turf, Dallas, Texas and Ardmore, Oklahoma, May 18-22, 2003
Source:Molecular Breeding of Forage and Turf: Proceedings of the 3rd International Symposium, Molecular Breeding of Forage and Turf. 2004, p. 245-254.
# of Pages:10
Publishing Information:Dordrecht: Kluwer Academic Publishers
Series:Developments in Plant Breeding Volume 11
Keywords:TIC Keywords: Transgenes; Genetic engineering; Cultivar improvement; Reproductive sterility; Herbicide resistance; Gene flow
Abstract/Contents:"Trait improvement of turfgrass through genetic engineering is important to the turfgrass industry and the environment. However, the possiblility of transgene escape to wild and non-transformed species raises commercial and ecological concerns. Male sterility provides an effective way for interrupting gene flow. We have designed and synthesized two chimeric gene constructs consisting of a rice tapetum-specific promoter (TAP) fused to either a ribonuclease gene barnase, or the antisense of a rice tapetum-specific gene rts. Both constructs were linked to the bar gene for selection by resistance to the herbicide glufosinate. Using Agrobacterium-mediated transformation, we have successfully introduced those gene constructs into creeping bentgrass (cv Penn-A-4), producing a total of 219 stably transformed individual events. Tapetum-specific expression of barnase or antisense rts gene did not effect the vegetative phenotype compared with the control plants, and male-sterile flowers were obtained with both constructs. Microscopic studies confirmed the failure of mature pollen formation in male-sterile transgenics. Mendelian segregation of herbicide tolerance and male sterility has been observed in T1 progeny derived from crosses with wild-type plants. Male sterility in transgenic grasses provides the best tool to evaluate gene flow in genetically modified perennial plants and should facilitate the application of genetic engineering in producing environmentally responsible grasses with enhanced traits."
ISBN:1-4020-1867-3
Language:English
References:42
Note:Figures
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Luo, H., Q. Hu, K. Nelson, C. Longo, and A. P. Kausch. 2004. Controlling transgene escape in genetically modified grasses. Molecular Breeding of Forage and Turf: Proceedings of the 3rd International Symposium, Molecular Breeding of Forage and Turf. p. 245-254.
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