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| DOI: | 10.1007/s11032-005-4784-8 |
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| Web URL(s): | http://www.springerlink.com/openurl.asp?genre=article&issn=1380-3743&volume=16&issue=2&spage=185
Last checked: 12/2005
Access conditions: Item is within a limited access website |
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| Publication Type:
| Refereed |
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| Author(s): | Luo, Hong;
Kausch, Albert P.;
Hu, Qian;
Nelson, Kimberly;
Wipff, Joseph K.;
Fricker, Crystal C. R.;
Owen, T. Page;
Moreno, Maria A.;
Lee, Jang-Yong;
Hodges, Thomas K. |
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| Author Affiliation: | Luo, Kausch, Hu, Nelson and Hodges: HybriGene Inc., West Kingston, Rhode Island; Luo and Kausch: Department of Cell and Molecular Biology, University of Rhode Island, Kingston, Rhode Island; Wipff anf Fricker: Pure Seed Testing Inc., Hubbard, Oregon; Owen: Department of Botany, Connecticut College, New London, Connecticut; Moreno: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut; Lee: National Institute of Crop Science, Suwon, Korea |
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| Title: | Controlling transgene escape in GM creeping bentgrass |
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| Source: | Molecular Breeding. Vol. 16, No. 2, September 2005, p. 185-188. |
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| Publishing Information: | The Netherlands: Kluwer Academic Publishers |
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| # of Pages: | 4 |
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| Keywords: | TIC Keywords: Transgenes; Transgenic turfgrasses; Agrostis stolonifera; Genetic drift; Reproductive sterility; Promoters; Herbicide resistance
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| Cultivar Names: | Penn A-4 |
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| Abstract/Contents: | "Trait improvement of turfgrass through genetic engineering is important to the turfgrass industry and the environment. However, the possible transgene escape to wild and non-transformed species raises ecological and commercial concerns. Male sterility provides an effective way for interrupting gene flow. We have designed and synthesized two chimeric gene constructs consisting of a rice tapetum-specific promoter (TAP) fused to either a ribonuclease gene barnase, or the antisense of a rice tapetum-specific gene rts. Both constructs were linked to the bar gene for selection by resistance to the herbicide glufosinate. Agrobacterium-mediated transformation of creeping bentgrass (cv Penn A-4) with both constructs resulted in herbicide-resistant transgenic plants that were also 100% pollen sterile. Mendelian segregation of herbicide resistance and male sterility was observed in T1 progeny derived from crosses with wild-type plants. Controlled self- and cross-pollination studies showed no gene transfer to non-transgenic plants from male-sterile transgenic plants. Thus, male sterility can serve as an important tool to control transgene escape in bentgrass, facilitating the application of genetic engineering in producing environmentally responsible turfgrass with enhances traits. It also provides a tool to control gene flow in other perennial species using transgenic technology." |
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| Language: | English |
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| References: | 17 |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Luo, Hong, Albert P. Kausch, Qian Hu, Kimberly Nelson, J. K. Wipff, C. C. R. Fricker, et al. 2005. Controlling transgene escape in GM creeping bentgrass. Molecular Breeding. 16(2):p. 185-188. |
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| DOI: 10.1007/s11032-005-4784-8 |
| Web URL(s):
http://www.springerlink.com/openurl.asp?genre=article&issn=1380-3743&volume=16&issue=2&spage=185
Last checked: 12/2005
Access conditions: Item is within a limited access website |
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