Full TGIF Record # 1087
Item 1 of 1
DOI:10.1016/S0953-7562(09)80488-7
Web URL(s):https://www.sciencedirect.com/science/article/pii/S0953756209804887?via%3Dihub
    Last checked: 10/08/2019
    Access conditions: Item is within a limied-access website
Publication Type:
i
Refereed
Author(s):Henson, Joan M.
Author Affiliation:Department of Microbiology, Montana State University, Bozeman, Montana
Title:DNA hybridization and polymerase chain reaction (PCR) tests for identification of Gaeumannomyces, Phialophora and Magnaporthe isolates
Source:Mycological Research. Vol. 96, No. 8, August 1992, p. 629-636.
Publishing Information:Cambridge University Press
# of Pages:8
Related Web URL:http://www.sciencedirect.com/science/article/pii/S0953756209804887
    Last checked: 06/09/2015
    Notes: Abstract only
Keywords:TIC Keywords: DNA; Hybridization; Disease identification; Gaeumannomyces; Phialophora; Magnaporthe poae; Take-all patch
Abstract/Contents:"DNA hybridization and PCR tests for Gaeumannomyces graminis and related fungi were developed to aid in their identification and classification. Hybridizations with a Gaeumannomyces graminis var. tritici mitochondrial DNA probe to restriction fragments from other Gaeumannomyces, Phialophora or Magnaporthe isolates demonstrated that almost all of the tested Gaeumannomyces and Phialophora isolates, including Gaeumannomyces cylindrosporus and Gaeumannomyces incrustans, had sequences homologous to the probe. In addition M. poae had strong homology with the probe. Most isolates of Gaeumannomyces graminis var. tritici, the causative agent of take-all disease of cereals, had identical EcoR I and Msp I hybridization patterns, whereas sizes of hybridizing restriction fragments of Phialophora and Magnaporthe isolates showed more variation. Polymerase chain reaction (PCR) tests with primers developed for G. graminis var. tritici were positive for all G. graminis isolates. PCR tests for G. incrustans, G. cylindrosporus, Phialophora and Magnaporthe were positive, but test conditions in which positive results were obtained were more limited than PCR conditions which amplified G. graminis DNA."
Language:English
References:28
Note:Pictures, b/w
Tables
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Henson, J. M. 1992. DNA hybridization and polymerase chain reaction (PCR) tests for identification of Gaeumannomyces, Phialophora and Magnaporthe isolates. Mycol. Res. 96(8):p. 629-636.
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DOI: 10.1016/S0953-7562(09)80488-7
Web URL(s):
https://www.sciencedirect.com/science/article/pii/S0953756209804887?via%3Dihub
    Last checked: 10/08/2019
    Access conditions: Item is within a limied-access website
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MSU catalog number: QK 600. B6
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