Full TGIF Record # 109341
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Web URL(s):http://turf.rutgers.edu/research/abstracts/symposium2006.pdf#page=44
    Last checked: 11/26/2007
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Publication Type:
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Report
Content Type:Abstract or Summary only
Author(s):Rotter, David; Bonos, Stacy A.; Meyer, William A.; Warnke, Scott; Belanger, Faith C.
Author Affiliation:Rotter, Bonos, Meyer, and Belanger: Department of Plant Biology and Pathology, Rutgers University; Warnke: United States Department of Agriculture, Beltsville, Maryland;
Title:Colonial bentgrass genetic linkage mapping
Section:Posters
Other records with the "Posters" Section
Meeting Info.:Cook College, Rutgers, NJ: January 12-13, 2006
Source:Proceedings of the Fifteenth Annual Rutgers Turfgrass Symposium. 2006, p. 43.
Publishing Information:New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey
# of Pages:1
Keywords:TIC Keywords: Agrostis stolonifera; Gene mapping; Dollar spot; Disease resistance; Genetic resistance; Hybrids
Abstract/Contents:"Creeping bentgrass (Agrostis stolonifera L.), an important turfgrass species, is highly susceptible to dollar spot disease caused by the fungus Sclerotinia homoeocarpa F.T. Bennett. Colonial bentgrass (A. capillaris L.) is a closely related species that generally has good dollar spot resistance. Interspecific hybrids between creeping and colonial bentgrass have been made and several hybrids exhibited excellent resistance to dollar spot (Belanger et al., 2004). One hybrid in particular, named TH15, had excellent resistance, as well as many desirable morphological traits. This plant was crossed with a creeping bentgrass plant to create a backcross population, which exhibited a wide range of phenotypical traits. Some of the individuals exhibited excellent dollar spot resistance originating from the colonial bentgrass genome. We are interested in identifying the genetic basis of dollar spot resistance in colonial bentgrass. Our approach is to generate a genetic linkage map of colonial bentgrass using this backcross population. By identifying colonial specific markers and tracking their segregation in this population we can use the colonial component of the hybrid genome to create a genetic linkage map of colonial bentgrass. Expressed sequence tag (EST) libraries of creeping and colonial bentgrass have been made and have been made functionally characterized. Using an interspecific hybrid and our backcross population for mapping provides a unique opportunity to take advantage of this sequence between colonial and creeping bentgrass homologous sequences. These SNPs can be evaluated by a technique known as minisequencing. This involves a single base extension of a polymorphic locus by using a fluorescent dideoxynucleotide. The resulting labled primer can be visualized either by polyacrylamide gel or DNA sequencer. Using this method we can follow the inheritance of colonial bentgrass genes, as identified by colonial bentgrass SNPs, in the mapping population. This technique is currently being used in combination with other gene based and AFLP based markers to create a genetic linkage map of colonial bentgrass."
Language:English
References:1
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Rotter, D., S. A. Bonos, W. A. Meyer, W. A. Meyer, S. Warnke, and F. C. Belanger. 2006. Colonial bentgrass genetic linkage mapping. Proc. Annu. Rutgers Turfgrass Symp. p. 43.
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http://turf.rutgers.edu/research/abstracts/symposium2006.pdf#page=44
    Last checked: 11/26/2007
    Requires: PDF Reader
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MSU catalog number: SB 433 .R88
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