Full TGIF Record # 10945
Item 1 of 1
Publication Type:
i
Refereed
Author(s):Robertson, Albert J.; Gusta, Lawrence V.; Reaney, Martin J.; Ishikawa, Masaya
Author Affiliation:Crop Development Center, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Title:Protein Synthesis in Bromegrass (Bromus inermis Leyss) Cultured Cells During the Induction of Frost Tolerance by Abscisic Acid or Low Temperature
Source:Plant Physiology. Vol. 84, No. 4, August 1987, p. 1331-1336.
Publishing Information:Lancaster, PA: American Society of Plant Physiologists
Keywords:TIC Keywords: Bromus inermis; Abscisic acid; Frost resistance
Abstract/Contents:"Bromus inermis Leyss cell cultures treated with 75 micromolar abscisic acid (ABA) at both 23 and 3 degrees C developed more freezing resistance than cells cultured at 3 degrees C. Protein synthesis in cells induced to become freezing tolerant by ABA and low temperature was monitored by [14 C] leucine incorporation. Protein synthesis continued at 3 degrees C, but net cell growth was stopped. Most of the major proteins detected at 23 degrees C were synthesized at 3 degrees C. However, some proteins were synthesized only at low temperatures, whereas others were inhibited. ABA showed similar effects on protein synthesis at both 23 and 3 degrees C. Comparative electrophoretic analysis of [14 C] leucine labeled protein detected the synthesis of 19, 21 and 47 kilodalton proteins in less than 8 hours after exposure to exogenous ABA. Proteins in the 20 kilodalton range were also synthesized at 3 degrees C. In addition, a 31 kilodalton protein band showed increased expression in freezing resistant ABA treated cultures after 36 hours growth at both 3 and 23 degrees C. Quantitative analysis of [14C] leucine labeled polypeptides in two-dimensional gels confirmed the increased expression of the 31 kilodalton protein. Two-dimensional analysis also resolved a 72 kilodalton protein enriched in ABA treated cultures and identified three proteins (24.5, 47, and 48 kilodaltons) induced by low temperature growth."
Language:English
References:28
Note:Figures
Tables
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Robertson, A. J., L. V. Gusta, M. J. Reaney, and M. Ishikawa. 1987. Protein Synthesis in Bromegrass (Bromus inermis Leyss) Cultured Cells During the Induction of Frost Tolerance by Abscisic Acid or Low Temperature. Plant Physiol. 84(4):p. 1331-1336.
Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=10945
If there are problems with this record, send us feedback about record 10945.
Choices for finding the above item:
Find Item @ MSU
MSU catalog number: QK 1 .P68
Request through your local library's inter-library loan service (bring or send a copy of this TGIF record)