| |
Publication Type:
| Refereed |
Author(s): | Robertson, Albert J.;
Gusta, Lawrence V.;
Reaney, Martin J.;
Ishikawa, Masaya |
Author Affiliation: | Crop Development Center, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. |
Title: | Protein Synthesis in Bromegrass (Bromus inermis Leyss) Cultured Cells During the Induction of Frost Tolerance by Abscisic Acid or Low Temperature |
Source: | Plant Physiology. Vol. 84, No. 4, August 1987, p. 1331-1336. |
Publishing Information: | Lancaster, PA: American Society of Plant Physiologists |
Keywords: | TIC Keywords: Bromus inermis; Abscisic acid; Frost resistance
|
Abstract/Contents: | "Bromus inermis Leyss cell cultures treated with 75 micromolar abscisic acid (ABA) at both 23 and 3 degrees C developed more freezing resistance than cells cultured at 3 degrees C. Protein synthesis in cells induced to become freezing tolerant by ABA and low temperature was monitored by [14 C] leucine incorporation. Protein synthesis continued at 3 degrees C, but net cell growth was stopped. Most of the major proteins detected at 23 degrees C were synthesized at 3 degrees C. However, some proteins were synthesized only at low temperatures, whereas others were inhibited. ABA showed similar effects on protein synthesis at both 23 and 3 degrees C. Comparative electrophoretic analysis of [14 C] leucine labeled protein detected the synthesis of 19, 21 and 47 kilodalton proteins in less than 8 hours after exposure to exogenous ABA. Proteins in the 20 kilodalton range were also synthesized at 3 degrees C. In addition, a 31 kilodalton protein band showed increased expression in freezing resistant ABA treated cultures after 36 hours growth at both 3 and 23 degrees C. Quantitative analysis of [14C] leucine labeled polypeptides in two-dimensional gels confirmed the increased expression of the 31 kilodalton protein. Two-dimensional analysis also resolved a 72 kilodalton protein enriched in ABA treated cultures and identified three proteins (24.5, 47, and 48 kilodaltons) induced by low temperature growth." |
Language: | English |
References: | 28 |
Note: | Figures Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Robertson, A. J., L. V. Gusta, M. J. Reaney, and M. Ishikawa. 1987. Protein Synthesis in Bromegrass (Bromus inermis Leyss) Cultured Cells During the Induction of Frost Tolerance by Abscisic Acid or Low Temperature. Plant Physiol. 84(4):p. 1331-1336. |
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