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DOI: | 10.2135/cropsci2005.04-0008 |
Web URL(s): | https://dl.sciencesocieties.org/publications/cs/articles/46/1/162 Last checked: 11/16/2016 Access conditions: Item is within a limited-access website https://dl.sciencesocieties.org/publications/cs/pdfs/46/1/162 Last checked: 11/16/2016 Requires: PDF Reader Access conditions: Item is within a limited-access website |
Publication Type:
| Refereed |
Author(s): | Rajasekar, Shanmugam;
Fei, Shui-hang;
Christians, Nick E. |
Author Affiliation: | Department of Horticulture, Iowa State University, Ames, Iowa |
Title: | Analysis of genetic diversity in rough bluegrass determined by RAPD markers |
Section: | Plant genetic resources Other records with the "Plant genetic resources" Section
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Source: | Crop Science. Vol. 46, No. 1, January/February 2006, p. 162-167. |
Publishing Information: | Madison, WI: Crop Science Society of America |
# of Pages: | 6 |
Related Web URL: | https://dl.sciencesocieties.org/publications/cs/abstracts/46/1/162 Last checked: 11/16/2016 Notes: Abstract only |
Keywords: | TIC Keywords: Poa trivialis; Genetic diversity; Genetic variability; Random amplified polymorphic DNA markers; Analysis of molecular variance
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Abstract/Contents: | "Information on genetic variation in rough bluegrass (Poa trivialis L.), a cool-season grass that is grown for sports fields and lawns, is needed. The objective of this study was to access genetic variaion in 27 accessions of rough bluegrass obtained from the National Plant Germplasm System (NPGS) by random amplified polymorphic DNA (RAPD) markers. Fifteen of the 80 primers screened generated 64 highly repeatable polymorphic bands. A dendrogram constructed on the basis of the Unweighted Pair Group Method with Arithmetic Average (UPGMA) clustering algorithm revealed that 26 of 27 accessions formed two distinct clusters. Genetic similarity coefficients calculated from the RAPD data ranged from 0.07 to 0.74 with the lowest value of 0.07 measured between PI 254908, PI 594396, PI 250982, and PI 229782 from Iraq, the USA, Yugoslavia, and Iran, respectively. The highest value of 0.74 was measured between p1 225826, P1 289643, and P1 592521 from Denmark, Spain and the USA, respectively. The cophenetic correlation coefficient (r) was 0.90, indicative of a very good fit between the data matrix and the resulting cluster analysis. Principle coordinate analysis (PCO) clearly grouped 26 accessions on the two axes, with a single accession that did not cluster with the others. The PCO clustering pattern corresponded well with the dendogram. PI 254908 from Iraq did not cluster with any other accessions, either in the dendogram or on the basis of the PCO." |
Language: | English |
References: | 29 |
Note: | Figures Tables Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Christians, N. E., S. Rajasekar, and S. Fei. 2006. Analysis of genetic diversity in rough bluegrass determined by RAPD markers. Crop Sci. 46(1):p. 162-167. |
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| DOI: 10.2135/cropsci2005.04-0008 |
| Web URL(s): https://dl.sciencesocieties.org/publications/cs/articles/46/1/162 Last checked: 11/16/2016 Access conditions: Item is within a limited-access website https://dl.sciencesocieties.org/publications/cs/pdfs/46/1/162 Last checked: 11/16/2016 Requires: PDF Reader Access conditions: Item is within a limited-access website |
| MSU catalog number: SB 183 .C7 |
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