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DOI: | 10.21273/JASHS.130.4.619 |
Web URL(s): | https://journals.ashs.org/jashs/view/journals/jashs/130/4/article-p619.xml?rskey=fVYRgH Last checked: 11/10/2019 Requires: PDF Reader |
Publication Type:
| Refereed |
Author(s): | Salehi, Hassan;
Seddighi, Zahra;
Kravchenko, Alexandra N.;
Sticklen, Mariam B. |
Author Affiliation: | Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan |
Title: | Expression of the cry1Ac in "Arizona Common" common bermudagrass via Agrobacterium- mediated transformation and control of black cutworm |
Section: | Molecular biology-bioteechnology Other records with the "Molecular biology-bioteechnology" Section
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Source: | Journal of The American Society for Horticultural Science. Vol. 130, No. 4, July 2005, p. 619-623. |
Publishing Information: | Alexandria, VA: American Society for Horticultural Science |
# of Pages: | 5 |
Keywords: | TIC Keywords: Cynodon dactylon; Agrotis ipsilon; Golf greens; Calli; Genetic transformation; Bacillus thuringiensis; Genetic resistance; Insect resistance
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Cultivar Names: | Arizona common |
Abstract/Contents: | "Bermudagrass (Cynodon L.C. Rich.) is grown on more than 4 million ha in the southern United States. The black cutworm (Agrotis ipsilon Hufnagel) is the most commonly encountered pest of bermudagrass, especially on golf course greens. Developing insect-resistant cultivars is a very desirable substitute, both environmentally and economically, to using current synthetic pesticides. Here we report, for the first time, Agrobacterium-mediated transformation of "Arizona Common" common bermudagrass [Cynodon dactylon (L.) Pers.] with the Bacillus thuringiensis Berliner cry1Ac gene encoding an endotoxin active against black cutworm. Mature seeds were used for producing embryogenic callus, and calli were transformed with a plasmid containing a synthetic cry1Ac and the kanamycin resistance (nptII) genes. Putative transgenic calli and plantlets were selected on media containing 100 and 50 mg· L-1 G418, respectively. RNA-blot analysis of PCR-positive lines revealed the expression of the cry1Ac transgene in three out of five putative transgenic lines. The larvae fed on transgenic plant leaves experienced highly significant (over 80%) mortality." |
Language: | English |
References: | 33 |
Note: | Pictures, b/w Figures Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Salehi, H., Z. Seddighi, A. N. Kravchenko, and M. B. Sticklen. 2005. Expression of the cry1Ac in "Arizona Common" common bermudagrass via Agrobacterium- mediated transformation and control of black cutworm. J. Am. Soc. Hortic. Sci. 130(4):p. 619-623. |
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| DOI: 10.21273/JASHS.130.4.619 |
| Web URL(s): https://journals.ashs.org/jashs/view/journals/jashs/130/4/article-p619.xml?rskey=fVYRgH Last checked: 11/10/2019 Requires: PDF Reader |
| MSU catalog number: SB 1 .A46 |
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