Full TGIF Record # 11919
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Web URL(s):http://www.apsnet.org/publications/phytopathology/backissues/Documents/1988Articles/Phyto78n02_237.pdf
    Last checked: 10/15/2015
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Publication Type:
i
Refereed
Author(s):Saha, D. C.; Jackson, M. A.; Johnson-Cicalese, J. M.
Author Affiliation:Dept. of Soils and Crops, New Jersey Agricultural Experiment Station, New Brunswick, NJ.
Title:A Rapid Staining Method for Detection of Endophytic Fungi in Turf and Forage Grasses
Source:Phytopathology. Vol. 78, No. 2, February 1988, p. 237-239.
Publishing Information:St. Paul, MN: American Phytopathological Society
Keywords:TIC Keywords: Techniques; Endophytic fungi; Disease identification
Abstract/Contents:"Because of the increased interest in endophyte-infected grasses, an efficient detection method was deemed necessary. To facilitate screening a large number of individual seeds and plants, a rapid, safe staining procedure was developed using rose bengal. Previous methods were time-consuming and often required careful boiling of the plant tissues. The rose bengal staining prcedure is simple and effective and gave excellent visual results. Minimal staining times of 30-60 sec gave good fungal differentiation even in thick tissues, and no additional fixing was necessary. Seeds and dried tissue also were stained effectively."
Language:English
References:12
Note:Pictures: Color
Tables
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Saha, D. C., M. A. Jackson, and J. M. Johnson-Cicalese. 1988. A Rapid Staining Method for Detection of Endophytic Fungi in Turf and Forage Grasses. Phytopathology. 78(2):p. 237-239.
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Web URL(s):
http://www.apsnet.org/publications/phytopathology/backissues/Documents/1988Articles/Phyto78n02_237.pdf
    Last checked: 10/15/2015
    Requires: PDF Reader
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MSU catalog number: SB 599 .P48
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