Full TGIF Record # 12211
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Web URL(s):http://www.jstor.org/stable/2433294?seq=1#page_scan_tab_contents
    Last checked: 12/17/2015
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Publication Type:
i
Refereed
Author(s):Lawton, June R.
Author Affiliation:Electron Microscope Unit, University of Durban-Westville, P Bag X54001, Curban 4000, South Africa.
Title:Ultrastructure of Chloroplast Membranes in Leaves of Maize and Ryegrass as Revealed by Selective Staining Methods
Source:New Phytologist. Vol. 108, No. 3, March 1988, p. 277-283.
Publishing Information:Oxford, England
Keywords:TIC Keywords: Chloroplasts; Lolium multiflorum; Zea mays; Staining
Abstract/Contents:SUMMARY: "Leaves of Lolium multiflorum Lam. and Zea mays L. of different ages were prepared for TEM by a number of cytochemical methods. After post-fixation in potassium ferricyanide with osmium totroxide the peripheral reticulum in both mesophyll and bundle sheath chloroplasts of maize stain very clearly. Post-fixation with uranyl acetate, copper and lead salts also stains the chloroplast envelope rather than the internal thylakoid system. Differences between themesophyll and bundle sheath chloroplasts of maize and between those of ryegrass are revealed after post-fixation in zinc iodide with osmium tetroxide. After post-fixation in potassium iodide with osmium tetroxice the loculi stain less intensely than in zinc iodide. Malechite green addded to glutaraldehyde at pH 7.0 enhances membrane staining in general and reveals large lipid drips in the stroma. At pH below 6.0 only the 'A' space is stained. The 'A' space and the loculus are stained when ethidium bromide is added to glutaraldehyde. The different reactions of the chloroplast membranes probably reflect differences in chemical composition."
Language:English
References:22
Note:Figures
Tables
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Lawton, J. R. 1988. Ultrastructure of Chloroplast Membranes in Leaves of Maize and Ryegrass as Revealed by Selective Staining Methods. New Phytol. 108(3):p. 277-283.
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Web URL(s):
http://www.jstor.org/stable/2433294?seq=1#page_scan_tab_contents
    Last checked: 12/17/2015
    Access conditions: Item is within a limited-access website
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