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| DOI: | 10.1017/S0953756296002602 |
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| Web URL(s): | https://www.sciencedirect.com/science/article/pii/S0953756208607339?via%3Dihub
Last checked: 10/08/2019
Access conditions: Item is within a limited access website |
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| Publication Type:
| Refereed |
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| Author(s): | Chung, K.-R.;
Schardl, C. L. |
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| Author Affiliation: | Department of Plant Pathology, University of Kentucky, Lexington, Kentucky |
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| Title: | Sexual cycle and horizontal transmission of the grass symbiont, Epichloë typhina |
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| Source: | Mycological Research. Vol. 101, No. 3, March 1997, p. 295-301. |
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| Publishing Information: | Cambridge University Press |
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| # of Pages: | 7 |
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| Related Web URL: | http://www.sciencedirect.com/science/article/pii/S0953756208607339
Last checked: 01/31/2014
Notes: Abstract only |
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| Keywords: | TIC Keywords: Sexual reproduction; Fertilization; Endophytes; Epichloe typhina; Mating disruption; Life cycle
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| Abstract/Contents: | "IEpichloë typhina is a biotrophic symbiont of grass with its sexual state on immature host inflorescences. The ectophytic, presexual structure (stroma) of E. typhina produces spermatia and also serves as the female structure in mating. After fertilization there is a proliferation of a dense mycelium which has been suggested to be heterokaryotic. Perithecia form in the thicked stroma. We investigated the possible formation of heterokayrons in matings and the role of ascospores in contagious spread of E. typhina. In almost all instances the transfer to a stroma of spermatia of oppistie mating type leads to a thicking of the stroma; however, ascospores were produced only if the parents were of the same mating population. Fertilization of part of the stroma by one spermatial parent often inhibited fertilization by another strain elsewhere on the stroma depending upon the spermatial isolated used. Tests for heterokaryon formation were preformed by culturing stromata after matings that produced no ascospores, to avoid analysing meiotic products. In two instances the female was cultured, and in two the nuclear and mitochondrial haplotypes were primarily the male. In another instance mitochondrial haplotypes and mitochondrial plasmids from the female became associated with the male nuclear haplotype. These results suggest that, following mating, male hyphae proliferate and heterokaryons may sometimes f rm and also proliferate. To test how ascospores mediate infection, inflorescences of uninfected perennial ryegrass plants were surrounded by fertilized stromata from which E. typhina ascospores were being ejected, seeds were collected and grown, newly infected progeny plants were identified, and isolated from five progeny plants were analysed genetically. As expected for ascospore progeny the rDNA haplotype of the stromal and spermatial parents had segregrated, whereas all five isolates had the stromal profile of mitochondrial DNA. This observation demonstrated that ascopores mediate infection of new host plants." |
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| Language: | English |
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| References: | 22 |
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| Note: | Pictures, b/w
Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Chung, K.-R., and C. L. Schardl. 1997. Sexual cycle and horizontal transmission of the grass symbiont, Epichloë typhina. Mycol. Res. 101(3):p. 295-301. |
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| DOI: 10.1017/S0953756296002602 |
| Web URL(s):
https://www.sciencedirect.com/science/article/pii/S0953756208607339?via%3Dihub
Last checked: 10/08/2019
Access conditions: Item is within a limited access website |
| 
MSU catalog number: QK 600. B6 |
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