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| Web URL(s): | http://turf.rutgers.edu/research/abstracts/symposium2007.pdf#page=39
Last checked: 11/26/2007
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Bonos, Stacy A.;
Cortese, Laura;
Crouch, JoAnne;
Weibel, Eric N.;
Miller, Christopher;
Skaradek, William |
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| Author Affiliation: | Bonos, Cortese, Crouch, and Weibel: Department of Plant Biology and Pathology, Rutgers University; Miller, and Skaradek: United States Department of Agriculture - Natural Resources Conservation Service, Cape May Plant Materials Center, Cape May, New Jersey |
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| Title: | Morphological and molecular analysis of fourteen switchgrass populations grown in New Jersey |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | Cook College, Rutgers, NJ: January 11-12, 2007 |
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| Source: | Proceedings of the Sixteenth Annual Rutgers Turfgrass Symposium. Vol. 16, 2007, p. 38-39. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 2 |
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| Keywords: | TIC Keywords: Panicum virgatum; Cultivar variation; Genetic diversity; Genetic markers; Morphology; Molecular genetics
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| Abstract/Contents: | "Although a significant amount of genetic diversity exists within switchgrass (Panicum virgatum), little research has been conducted on the level of genetic diversity and local adaptation among different populations/ecotypes currently recommended for habitat restoration and biofuel production in the northeastern US. Switchgrasses are divided into upland and lowland ecotypes. Upland ecotypes are shorter, finer stemmed and more adapted to drier habitats, while lowland ecotypes are coarse-stemmed, tall growing and more robust (Lewandowski et al., 2003). The objectives of this study were to determine molecular and morphological differences within and between 14 different switchgrass populations. Switchgrass seed from 14 populations was obtained from various sources. 'Carthage', 'Timber', 'Contract', 'Shelter' and 'High Tide' germplasm was obtained from the Natural Resources Conservation Service-United States Department of Agriculture Plant Materials Center in Cape May, NJ, and represented northeastern population. All additional germplasm ('Caddo', 'Shawnee', 196, Pav12, Turkey, 'Sunburst', 'Kanlow', 'Pathfinder', and 'Blackwell') was obtained from the Plant Introduction (PI) collection curated by the Germplasm Resources Information Network (GRIN) and included standard cultivars developed in the Midwest, and germplasm sources from other countries. Carthage, Contract, Shelter, High Tide, Caddo, Shawnee, Sunburst, Pathfinder and Blackwell are considered upland ecotypes, while, Timber and Kanlow are lowland ecotypes. Individual plants from each population were transplanted to a spaced-plant nursery in the spring of 2005 at the Rutgers University Plant Biology Research and Extension Farm at Adelphia, NJ. Morphological measurements were taken on 12 indiviuals from each of the 14 different switchgrass populations in 2005 and 2006. Measurements included plant and panicle height, and flag leaf height, length and width. Leaf tissue was collected from the same 12 individuals for molecular marker analysis. DNA was isolated from leaf tissue using the Sigma® GenElute™ Plant Genomic DNA Miniprep kit (Sigma-Aldrich Co., St. Louis, MO). Publicly available microsatellite (SSR) markers specific for switchgrass were utilized for molecular marker analysis (Tobias et al, 2006). Thirty-two SSR primer pairs were tested for polymorphism on the 12 individuals totaling 180 individual samples. SSR markers were genotyped on an ABI 3130 genetic analyzer. Morphological and marker data was analyzed using the program structure (Pritchard et al., 2000) which identifies clusters of related individuals from multilocus genotypes. The full data set was analyzed for all models from K=1 through 14. Significant morphological and molecular differences between switchgrass populations were observed. Structure analysis of morphological and molecular data separated the individuals into groups with the best distinction occurring for K=3. Based on morphological data from 2005 'Kanlow' and 'Timber' formed a unique group. These populations, both lowland ecotypes, looked phenotypically similar and were very tall stiff plants when compared to other populations evaluated. Structure also placed individuals from 'Pathfinder', 'Contract', and 'Blackwell' in a cluster. In addition, 'Caddo', 'Shawnee', 196, Pav12, Turkey, 'Sunburst' and 'Shelter' shared some similarities and were placed in a third class. Structure analysis of the 2006 morphological data produced similar results, as both 'Kanlow' and 'Timber' were assigned to one grouping. In contrast to the 2005 morphological data, 'Carthage' and 'High Tide', both northeastern ecotypes, were clustered together in 2006. The morphological analyses provided distinction between upland and lowland ecotypes, but did not completely separate northeastern from midwestern populations. Evaluation of molecular marker data using structure yielded different results than the morphological data analysis. Molecular marker data did not differentiate between upland or lowland ecotypes or between midwestern or northeastern populations, and was not sufficient to distinguish one population from another. One assigned group included 'Kanlow', 'Timber', and 'Contract'. In addition, 'Shawnee', 196, and 'Turkey' were clustered together, while 'Shelter and 'High Tide' were assigned to a third grouping. As such, continued work with molecular markers needed to further differentiate between switchgrass populations. Results were similar to findings of Casler et al. (2006), who reported high levels of within-population variability and a lack of genetic differentiation between bred switchgrass cultivars, prairie-remnant populations, and prairie-remnant seed increase cultivars." |
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| Language: | English |
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| References: | 4 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Bonos, S. A., L. Cortese, J. Crouch, E. N. Weibel, C. Miller, and W. Skaradek. 2007. Morphological and molecular analysis of fourteen switchgrass populations grown in New Jersey. Proc. Annu. Rutgers Turfgrass Symp. 16:p. 38-39. |
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| Web URL(s):
http://turf.rutgers.edu/research/abstracts/symposium2007.pdf#page=39
Last checked: 11/26/2007
Requires: PDF Reader |
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MSU catalog number: SB 433 .R88 |
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