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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Bunting, Tracy E.;
Plumley, Karen A.;
Clarke, Bruce B.;
Hillman, Bradley I. |
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| Author Affiliation: | Department of Plant Pathology, Rutgers University, New Brunswick, New Jersey |
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| Title: | Molecular cloning of Magnaporthe poae and identification of a useful detection probe |
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| Section: | Oral Presentations
Other records with the "Oral Presentations" Section
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| Meeting Info.: | Cook College, Rutgers, NJ: January 15-16, 1993 |
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| Source: | Proceedings of the Second Annual Rutgers TurfgrassSymposium. Vol. 2, 1993, p. 4. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Magnaporthe poae; Fungi; Summer patch; Clones; Genetic engineering
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| Abstract/Contents: | "Magnaporthe poae, the filamentous fungus that causes summer patch disease of turf, produces ectotrophic hyphae capable of infecting roots under hot, humid conditions. Definitive identification of the fungus can be difficult and time consuming due to the absence of distinguishing characteristics of the fungus in nature. We have initiated a project to produce a molecular probe that is useful for detecting and identifying the fungus. A partial library of genomic DNA from a virulent fungal strain has been cloned into the plasmid vector pGEM3Zf+ (Promega), and a complete genomic library has been cloned into the phage vector ΘFixII (Stratagene). Using 32P-labelled genomic DNA as a hybridization probe, several plasmid clones were identified that hybridized efficiently to M. poae DNA. One of these clones was selected for further study by Southern blot and limited sequence analysis for production of detection probes. Unlike M. grisea, the rice blast pathogen, we have no evidence for repeat DNA elements in M. poae that would be useful as pathogen-specific hybridization probes. Oligonucleotide primers capable of initiating amplification if a 450 bp fragment of DNA were synthesized and tested for their ability to function in control reactions with M. poae DNA and DNA from 15 other fungi associated with turfgrass roots. Only the plasmid control and M. poae DNA resulted in amplification of a product of the expected size, indicating that these primers appear to be useful for differentiating M. poae from other turfgrass pathogens, saprophytes, and endophytes. A fragment of the same size was amplified whether the imput DNA was extracted from highly virulent or less virulent isolates of the fungus. Experiments are in progress to determine whether PCR using these or other primers is a useful method for detection of M. poae under field conditions. The phage library will be used for molecular genetic experiments to identify genes of interest relating to the pathogenicity of M. poae." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Bunting, T. E., K. A. Plumley, B. B. Clarke, and B. I. Hillman. 1993. Molecular cloning of Magnaporthe poae and identification of a useful detection probe. Proc. Annu. Rutgers Turfgrass Symp. 2:p. 4. |
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MSU catalog number: SB 433 .R88 |
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