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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Lee, Lisa;
Day, Peter R.;
Tumer, Nilgun |
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| Author Affiliation: | Center for Agricultural Molecular Biology, Rutgers University, New Brunswick, New Jersey |
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| Title: | Manipulation of endophyte/turfgrass interaction |
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| Section: | Oral presentations
Other records with the "Oral presentations" Section
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| Meeting Info.: | Cook College, Rutgers, NJ: January 15-16, 1993 |
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| Source: | Proceedings of the Second Annual Rutgers TurfgrassSymposium. Vol. 2, 1993, p. 10. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Cultivar improvement; Endophytes; Germplasm; Poa; Agrostis; Poa pratensis; Agrostis stolonifera; Polymerase chain reaction; Random amplified polymorphic DNA markers
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| Abstract/Contents: | "Our program goals are: (a) to produce a germplasm collection of fungal endophyte-infected grasses concentrating on Poa and Agrostis species; (b) to produce a collection of unifungal endophyte cultures for classical and molecular analysis; (c) to extend the host range of endophytes to grasses where they have not been found; (d) to produce endophyte-specific DNA probes; (e) to use the probes to characterize endophyte variability and produce RAPD fingerprints for isolate identification; and (f) to develop turfgrass tissue culture regeneration and transformation system for the incorporation of agronomically important genes into turfgrass. After extensive screening of collections of Poa and Agrostis species throughout the United States and Europe (in collaboration with Dr. Jim White, Auburn University, Alabama), we have obtained a limited number of fungal endophyte-infected grasses in these two genera. However, as of now we did not find fungal endophytes in Kentucky bluegrass (Poa pratensis) and creeping bentgrass (Agrostis palustris). Therefore, we are currently developing an in vitro culture system for extending the host range of endophyte to Kentucky bluegrass and Creeping bentgrass. To date, a collection of unifungal endophyte cultures has been established and contains representative isolates from a wide variety of turfgrass genera. We are using mycelial macerates of these endophyte isolates to inoculate embryogenic callus lines of Poa pratensis and Agrostis palustris to obtain novel combinations of endophyte infected grasses. We have inoculated several embryogenic callus lines of creeping bentgrass with endophytes and successfully regenerated plants. Regenerating grasses were planted in the soil and are currently being examined for the presence of endophyte in the leaf bases. One Emerald bentgrass regenerate is positive. We produced fungal endophyte-specific DNA probes by the polymerase chain reaction (PCR) and diagnostic fingerprints of DNA sequences generated by randomly amplified polymorphic DNA (RAPD) PCR method. RAPD markers obtained with four ten base random DNA primers were successfully used to discriminate among individual endophyte isolates. A phylogenetic tree of endophyte isolates was generated using RAPD markers produced from 5 random primers. We have also developed tissue culture and regeneration systems for Kentucky bluegrass and Creeping bentgrass. The embryogenic callus lines established provide alternative tissues for creating endophyte containing grasses and target cells for the development of a stable transformation system by particle bombardment." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Lee, L., P. R. Day, and N. Tumer. 1993. Manipulation of endophyte/turfgrass interaction. Proc. Annu. Rutgers Turfgrass Symp. 2:p. 10. |
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MSU catalog number: SB 433 .R88 |
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