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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Zhang, Yongping;
Li, Taiyuan;
Metzler, Mary C.;
Chen, Tseh-An |
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| Author Affiliation: | Department of Plant Pathology, Rutgers University, New Brunswick, New Jersey |
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| Title: | Cloning a Bacillus thuringiensis crystal protein gene into Clavibacter xyli ssp. cynodontis |
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| Section: | Oral presentations
Other records with the "Oral presentations" Section
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| Meeting Info.: | Cook College, Rutgers, NJ: January 15-16, 1993 |
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| Source: | Proceedings of the Second Annual Rutgers TurfgrassSymposium. Vol. 2, 1993, p. 9. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Clones; Bacillus thuringiensis; Clavibacter xyli subsp. cynodontis; Bacteria; Genes; Escherichia coli; Genetic engineering
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| Abstract/Contents: | "A transformation system for Clavibacter xyli subsp. cynodontis (Cxc), a Gram-positive bacterium, was successful when using pLAFR3 as a vector, which was originally derived from a Gram-negative bacterium. The cloning of a crystal protein gene, cryIA(a), from Bacillus thuringiensis subsp. kurstaki Hdl-Dipel in Cxc was carried out by using pUC119 and pLAFR3. The recombinant DNA was transformed into Cxc by electroporation transformation. However, the gene did not express in either E. coli or Cxc transformants. It was then realized that the promoter of the gene was partially deleted and the gene is under the control of lacZ gene promoter. Recently another panel of recombinant plasmids were constructed, which cover the entire cryIA(a) gene and its upstream and downstream regions. Although the expression of the crystal gene in E. coli was relatively high, confirmed by western-blotting and northern blotting, the expression of the gene was not determined yet in Cxc since the recombinant DNA was very unstable in transformants, its loss rate about 13% per generation. The loss rate of the transformants with pLAFR3 alone is 1.5% per generation. Presently we are working on the native plasmid, pCXC100, from Cxc and hope to modify the plasmid and contstruct a vector system which is suitable for Cxc." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zhang, Y., T. Li, M. C. Metzler, and T.-A. Chen. 1993. Cloning a Bacillus thuringiensis crystal protein gene into Clavibacter xyli ssp. cynodontis. Proc. Annu. Rutgers Turfgrass Symp. 2:p. 9. |
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