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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Huff, David R.;
Peakall, Rod;
Smouse, Peter E. |
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| Author Affiliation: | Huff, and Peakall: Department of Plant Science; Smouse: Center for Theoretical and Applied Genetics, Rutgers University, New Brunswick, New Jersey |
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| Title: | RAPD variation in natural populations of outcrossing diploid buffalograss (Buchloë dactyloides) |
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| Section: | Oral presentations
Other records with the "Oral presentations" Section
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| Meeting Info.: | Cook College, Rutgers, NJ: January 15-16, 1993 |
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| Source: | Proceedings of the Second Annual Rutgers TurfgrassSymposium. Vol. 2, 1993, p. 13. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Random amplified polymorphic DNA markers; Diploids; Bouteloua dactyloides; Genetic variability; Semiarid climates; Polyploidy; Analysis of molecular variance; Population dynamics; Ecotypes; Cultivar identification
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| Abstract/Contents: | "RAPD markers provide a powerful tool for investigation of genetic variation in natural and domesticated populations that is simpler, less costly, and less labor intensive than other DNA marker methodologies. Recent studies of strain/cultivar identification have shown extensive RAPD divergence among but little variation within inbred species or cultivars. In contrast, little is known about the pattern and extent of RAPD variation in heterogeneous, outcrossing species. We describe the population genetic variation of RAPD markers in natural, diploid sources dioecious buffalograss (Buchloë dactyloides (Nutt.) Engelm.). Buffalograss is native to the semi-arid regions of the Great Plains of North America, where it is important for rangeland forage, soil conservation, and as turfgrass. Most sources of buffalograss germplasm are polyploid; diploid populations are only known from semi-arid Central Mexico. This is the first report of diploids from humid Gulf Coastal Texas. These two diploid sources represent divergent adaptive ecotypes. Seven 10-mer primers produced 98 polymorphic banding sites. Based on the presence/absence of bands, a genetic distance matrix was calculated. The new Analysis of Molecular Variance (AMOVA) technique was used to apportion the variation among individuals within populations, among populations within adaptive regions, and among regions. There was considerable variation within each of the four populations, and every individual was genetically distinct. Meaningful genetic divergence among local populations was found. Within-population variation was larger and among-population variation smaller in Mexico than in Texas. The genetic differences between the two regional ecotypes were categorical. These patterns of genetic variation were very different from those reported for inbred species and provide important baseline data for cultivar identification and continuing studies of the evolution of polyploid races for this species." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Huff, D. R., R. Peakall, and P. E. Smouse. 1993. RAPD variation in natural populations of outcrossing diploid buffalograss (Buchloë dactyloides). Proc. Annu. Rutgers Turfgrass Symp. 2:p. 13. |
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