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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Huff, David R.;
Bara, Janice M. |
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| Author Affiliation: | Department of Plant Science, Rutgers University, New Brunswick, New Jersey |
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| Title: | Determining genetic origins of aberrant progency from facultative apomictic Kentucky bluegrass using a combination of flow cytometry and RAPD markers |
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| Section: | Oral presentations
Other records with the "Oral presentations" Section
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| Meeting Info.: | Cook College, Rutgers, NJ: January 15-16, 1993 |
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| Source: | Proceedings of the Second Annual Rutgers TurfgrassSymposium. Vol. 2, 1993, p. 14. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Poa pratensis; Breeding; Apomixis; Progeny testing; Laser flow cytometry; Random amplified polymorphic DNA markers; Fertilization; Hybridization; Variations
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| Abstract/Contents: | "Kentucky bluegrass is an important agronomic crop with a facultative apomictic breeding system. Apomixis is an evolutionarily interesting and agronomically important means of asexual reproduction through seed. Facultative apomicts produce two types of progeny: apomictic progeny that are identical to the maternal genotype and aberrant progeny that are genetically different from the maternal genotype. Aberrant progeny have at least nine different genetic origins depending on gametic ploidy level and whether fertilization was self, cross, or absent. Multiple genetic origins of aberrant progeny complicate results of basic and applied genetic studies. Determining the genetic origins of progeny plants using the traditional techniques, such as cytology, embryology, and segregational studies is technically difficult in Kentucky bluegrass. We have found that two relatively new techniques, flow cytometry and RAPD markers, are powerful tools for rapidly determining the genetic origins of aberrant Kentucky bluegrass progeny. Our application of these techniques demonstrate that 1) flow cytometry accurately distinguishes progeny ploidy levels, and 2) RAPD markers distinguish progeny resulting from cross-fertilization. Therefore, a combination of flow cytometry and RAPD data would be useful for most genetic studies of aberrant individuals. Moreover, RAPDs were found to be useful for quantifying the loss of genetic markers from polyhaploids and the accumulation of genetic material in polydiploid BII (n+n) and polytriploid BIII (2n+n) hybrids. Quantifying shared RAPD markers will be useful for determining genetic relatedness between varieties and germplasm sources. The ability to distinguish the genetic origin of aberrant progeny, using flow-cytometry and RAPD markers, has powerful applications for more accurate descriptions of commercial varieties, for detecting hybridization events during early stages of a breeding program, and for genetic studies analysing variation of apomixis pathway components and the frequency of different genetic origins." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Huff, D. R., and J. M. Bara. 1993. Determining genetic origins of aberrant progency from facultative apomictic Kentucky bluegrass using a combination of flow cytometry and RAPD markers. Proc. Annu. Rutgers Turfgrass Symp. 2:p. 14. |
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