Full TGIF Record # 125453
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Publication Type:
i
Report
Content Type:Abstract or Summary only
Author(s):Hartman, Christine L.; Lee, Lisa; Day, Peter R.; Chin, Chee-kok; Zilinskas, Barbara; Tumer, Nilgun
Author Affiliation:Hartman, Lee, Day, and Tumer: Center for Agricultural Molecular Biology; Chin: Department of Horticulture; Zilinskas: Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, New Jersey
Title:Progress toward stable transformation and regeneration of Creeping bentgrass and Kentucky bluegrass
Section:Poster presentations
Other records with the "Poster presentations" Section
Meeting Info.:Cook College, Rutgers, NJ: January 15-16, 1993
Source:Proceedings of the Second Annual Rutgers TurfgrassSymposium. Vol. 2, 1993, p. 21.
Publishing Information:New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey
# of Pages:1
Keywords:TIC Keywords: Agrostis stolonifera; Poa pratensis; Herbicide resistance; Bialaphos; Superoxide dismutase
Abstract/Contents:"Our goal is to develop a high frequency turfgrass transformation system to produce stable transformants with single gene traits such as herbicide resistance, and improved oxidative stress tolerance. We have developed tissue culture and regeneration systems for Kentucky bluegrass and Creeping bentgrass. The embryogenic callus lines and suspension cultures established provide target cells for the development of a stable transformation system. Emerald and Putter bentgrass embryogenic callus lines have been used for transformation by particle bombardments. Transient assays using the E. coli ß - glucuronidase (GUS) reporter gene range from 100 - 1000 individual spots per plate showing expression. Selection for stable transformants has been through the use of the bar gene which confers resistance to the herbicide bialaphos. Concentrations of 2 or 4 mg/liter of bialaphos have been used for selection. Both plate and liquid selection have been attempted. Preliminary assays show GUS activity in roots and shoots of recovered plants. In addition, we have attempted to introduce the ascorbate peroxidase (AP) and superoxide dismutase (SOD) by co-transformation with selectable markers. These experiments are still in progress."
Language:English
References:0
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Hartman, C. L., L. Lee, P. R. Day, C.-k. Chin, B. Zilinskas, and N. Tumer. 1993. Progress toward stable transformation and regeneration of Creeping bentgrass and Kentucky bluegrass. Proc. Annu. Rutgers Turfgrass Symp. 2:p. 21.
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MSU catalog number: SB 433 .R88
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