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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Lam, Cuong;
Belanger, Faith;
White, James;
Daie, Jaleh |
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| Author Affiliation: | Lam, Belanger, and Daie: Plant Science Department, Rutgers University, New Brunswick, New Jersey; White: Department of Biology, Auburn University, Montgomery, Alabama |
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| Title: | Sugar uptake by choke-inducing and nonchoke-inducing isolates of Epichloe typhina /Acremonium typhinum |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | Cook College, Rutgers, NJ: January 15-16, 1993 |
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| Source: | Proceedings of the Second Annual Rutgers TurfgrassSymposium. Vol. 2, 1993, p. 22. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Epichloe typhina; Acremonium typhinum; Choke disease; Sugars; Endophytes; Fungi; Fructose; Sucrose; Glucose; Festuca rubra subsp. commutata; Festuca rubra subsp. rubra
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| Abstract/Contents: | "We are investigating the mechanisms of choke disease development in Festuca rubra. Sugar utilization capability of the fungal endophyte has been proposed as a factor important in the production of choke (Mycologia 83:601-610, 1991). We are monitoring sugar uptake in endophytes isolated from F. rubra cultivars which vary in their ability to induce choke disease. We have optimized culture conditions and developed a protocol for uptake experiments. To date we have monitored uptake of glucose, fructose, and sucrose by the endophytes isolated from two cultivars of F. rubra, Rainbow and England. The uptake rates for all three sugars were biphasic indicative of two distinct mechanisms of uptake. At low sugar concentrations the uptake was linear suggesting diffusion as the mechanism of uptake. At high sugar concentrations the uptake had the characteristics of an active, saturable, carrier-mediated mechanism. Glucose and sucrose uptake by the two isolates were essentially identical. The two cultivars differed reproducibly in their uptake of fructose. We have also done competition experiments where uptake of a labelled sugar was measured in the presence of varying concentrations of a different unlabelled sugar. Unlabelled sucrose did not compete with uptake of 14C-glucose and 14C-sucrose. From these results it appears that there are separate carriers for the three sugars. The specific carriers may also have weaker affinity for the other sugars. That unlabelled sucrose did not compete with 14C-glucose uptake suggests there was not cell wall invertase activity. The cultures used in these experiments were grown on glucose. We are currently measuring invertase activity directly with cultures grown on glucose or sucrose to determine if invertase may be induced when grown in the presence of sucrose. We are expanding our study to 10 fungal isolates from cultivars of Chewings fescue and strong creeping red fescue. These cultivars have had varying amounts of choke disease in the field in the past. In the spring of 1993 we will be quantifying the actual incidence of choke disease in these cultivars." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Lam, C., F. Belanger, J. White, and J. Daie. 1993. Sugar uptake by choke-inducing and nonchoke-inducing isolates of Epichloe typhina /Acremonium typhinum. Proc. Annu. Rutgers Turfgrass Symp. 2:p. 22. |
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