Full TGIF Record # 127253
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DOI:10.2135/cropsci2006.11.0699
Web URL(s):https://dl.sciencesocieties.org/publications/cs/pdfs/47/4/1691
    Last checked: 11/16/2016
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https://dl.sciencesocieties.org/publications/cs/articles/47/4/1691
    Last checked: 11/16/2016
    Access conditions: Item is within a limited-access website
Publication Type:
i
Refereed
Author(s):Sandhu, Sukhpreet; Altpeter, Fredy; Blount, Ann R.
Author Affiliation:Sandhu and Altpeter: Agronomy Department, Plant Molecular and Cellular Biology Program, Genetics Institute, University of Florida - Insitute of Food and Agricultural Sciences, Gainesville, Florida; Blount: North Florida Research and Education Center, Agronomy Department, University of Florida - Institute of Food and Agricultural Sciences, Marianna, Florida
Title:Apomictic bahiagrass expressing the bar gene is highly resistant to glufosinate under field conditions
Section:Genomics, molecular genetics & biotechnology
Other records with the "Genomics, molecular genetics & biotechnology" Section
Source:Crop Science. Vol. 47, No. 4, July/August 2007, p. 1691-1697.
Publishing Information:Madison, WI: Crop Science Society of America
# of Pages:7
Related Web URL:https://dl.sciencesocieties.org/publications/cs/abstracts/47/4/1691
    Last checked: 11/16/2016
    Notes: Abstract only
Keywords:TIC Keywords: Paspalum notatum; Glufosinate; Breeding improvement; Genetic transformation; Genetic engineering; Transgene expression; Weed control; Herbicide resistance; Calli; Genetic markers; Herbicide injury; Necrosis; Genetic resistance; Gene expression; Transgenic turfgrasses; Chloris
Abstract/Contents:"Bahiagrass (Paspalum notatum Flugge) is one of the most important low-input turf and forage grasses in the southeastern USA and in other subtropical regions. Its open growth habit, however, facilitates weed encroachment and its low tolerance to commercially available herbicides complicates weed management. Genetic transformation protocols were recently developed for bahiagrass that allow now, among other approaches, the introduction of herbicide resistance genes. Integration of the bar gene expression cassette into the genomic DNA of the apomictic bahiagrass cultivar Argentine was confirmed by Southern blot analysis. Stable expression of the bar gene was detected by an immunochromatographic assay in primary transgenic lines and the seed-derived progeny plants. Several independent transgenic lines showed no growth inhibition, chlorosis, or necrosis following a spray application of 1.0% glufosinate ammonium [2-amino-4-(hydroxymethylphosphinyl)butanoic acid] under greenhouse conditions. The selected transgenic plants did not differ morphologically from wild-type plants and produced viable seeds in the greenhouse and field. All weeds that coestablished during the field experiment, as well as wild-type bahiagrass, displayed full leaf necrosis after application of 0.3% glufosinate ammonium. Transgenic bahiagrass plants were resistant to a field application of 0.6% glufosinate ammonium, which is twice the recommended rate for weed control, without any symptoms of phytotoxicity."
Language:English
References:32
Note:Pictures, color
Figures
Tables
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Sandhu, S., F. Altpeter, and A. R. Blount. 2007. Apomictic bahiagrass expressing the bar gene is highly resistant to glufosinate under field conditions. Crop Sci. 47(4):p. 1691-1697.
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DOI: 10.2135/cropsci2006.11.0699
Web URL(s):
https://dl.sciencesocieties.org/publications/cs/pdfs/47/4/1691
    Last checked: 11/16/2016
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
https://dl.sciencesocieties.org/publications/cs/articles/47/4/1691
    Last checked: 11/16/2016
    Access conditions: Item is within a limited-access website
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