Full TGIF Record # 127652
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Web URL(s):https://gsrpdf.lib.msu.edu/?file=/2000s/2007/070914.pdf
    Last checked: 01/26/2017
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Author(s):Anderson, Michael P.; Wu, Yanqi
Author Affiliation:Anderson: Associate Professor; Wu: Assistant Professor, Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, Oklahoma
Title:Bermudagrass DNA fingerprinting: This powerful tool can be used to distinguish genetic differences that are important in protecting plant patents
Section:Research you can use
Other records with the "Research you can use" Section
Source:USGA Green Section Record. Vol. 45, No. 5, September/October 2007, p. 14-17.
Publishing Information:Far Hills, NJ: United States Golf Association, Green Section
# of Pages:4
Keywords:TIC Keywords: Cynodon; DNA; DNA amplification; Polymerase chain reaction; Genetics; Genetic variability; Varietal registration
Abstract/Contents:Discusses the use of DNA fingerprinting to identify genetic differences in bermudagrasses. Details the process of DNA identification, stating that "most DNA fingerprinting depends on a technique known as PCR or polymerase chain reaction. PCR was developed in the mid-[1980s] to efficiently amplify specific segments of DNA many-fold. The PCR technique uses short DNA segments composed of anywhere from 6 to 20 nucleotides known as primers, which are complementary to segments of the target DNA. The primers figuratively scan for matches in the target DNA sequences. Once a match is found, then amplification of that segment begins. If there are many matches, many segments will be amplified." Describes DNA fingerprinting research in bermudagrass, stating that "some of the first work highlighted the differences among high-quality commercial cultivars and select bermudagrasses found in germplasm collections." Reports that "research underscored the need for evaluating...varieties for genetic stability and purity. In addition...research...has discovered a few...discrepancies between the historical pedigree claims of several varieties and their actual genetic relationship using fingerprinting techniques." States that "DNA fingerprinting can have an impact in the area of patent protection. Many years of effort are expended to develop commercial varieties...To support the patent application process, differences in morphology, cultural characteristics, and pedigree need to be presented in order to distinguish the proposed variety from those that are...available." Concludes that "DNA fingerprinting technology remains a powerful technique to assess the genetic diversity of bermudagrasses worldwide and to protect plant varieties from infringement. At OSU [Oklahoma State University]...projects have been involved in using DNA fingerprinting to further bermudagrass improvement."
See Also:See also complete version in USGA Turfgrass and Environmental Research Online, 6(14) July 15 2007, p. [1-8] R=125651 R=125651
Note:Includes sidebar, ("Connecting the dots: An interview with Drs. Michael Anderson and Yanqi Wu regarding DNA fingerprinting", p. 17 by Dr. Jeff Nus) p. 17
Pictures, color
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Anderson, M. P., and Y. Wu. 2007. Bermudagrass DNA fingerprinting: This powerful tool can be used to distinguish genetic differences that are important in protecting plant patents. USGA Green Sec. Rec. 45(5):p. 14-17.
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    Last checked: 01/26/2017
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MSU catalog number: SB 433.15 .U84
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