Full TGIF Record # 127839
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Web URL(s):http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2007.97.7.S1#page=77
    Last checked: 10/20/2015
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Publication Type:
i
Report
Content Type:Abstract or Summary only
Author(s):Miller, G. L.; Tredway, L. P.
Author Affiliation:Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina
Title:Isolation and identification of the basidiomycete fungi causing fairy rings in golf course putting greens
Meeting Info.:APS Annual Meeting, San Diego, California: July 28 - August 1, 2007
Source:Phytopathology. Vol. 97, No. 7, July Supplement 2007, p. S77.
Publishing Information:St. Paul, MN: American Phytopathological Society
# of Pages:1
Keywords:TIC Keywords: Basidiomycota; Fairy rings; Golf greens; Fungi; Disease evaluation
Abstract/Contents:"Identification of the fungi responsible for causing fairy ring symptoms rests solely on basidocarp formation, which often does not occur. Samples from the soil and thatch layers of 4 bentgrass and 2 bermudagrass golf course putting greens exhibiting fairy ring symtpoms were collected. Several isolation techniques were evaluated, including the traditional soil block and soil sprinkle methods. A recently described method involving spore washing and the use of a lignin-benomyl-guaiacol selective media was also attempted. Toothpicks or other wooden substrates were also used as baits. Isolated mycelia were examined for the presence of clamp connections or identifiable asexual or sexual structures. Genomic DNA was extracted from putative basidomycetes, the ITS1 and ITS2 regions were sequenced,and the basidiomycete-specific primers 1f and 4b were also evaluated. No basidiomycetes were isolated from 5 of the 6 sampled soils, including one creeping bentgrass and one bermudagrass site where Lycoperdon perlatum puffballs were later associated with symptoms. A sterile white fungus with clamp connections was routinely isolated from one bermudagrass site with all of the methods attempted. The ITS 1f/4b primers selectively identified these isolates as basidiomycetes, and BLAST analysis of the region showed greatest homology to Marasmius oreades and other Marasmius species."
Language:English
References:0
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Miller, G. L., and L. P. Tredway. 2007. Isolation and identification of the basidiomycete fungi causing fairy rings in golf course putting greens. Phytopathology. 97(7):p. S77.
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Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2007.97.7.S1#page=77
    Last checked: 10/20/2015
    Requires: PDF Reader
    Notes: Item is within a single large file
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