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Web URL(s): | http://img.kisti.re.kr/soc_img/society//tsk/JDHHBF/1990/v4n1/JDHHBF_1990_v4n1_12.pdf Last checked: 01/28/2013 Requires: PDF Reader |
Publication Type:
| Refereed |
Author(s): | Kim, Byeong-Kiu;
Chang, Nam-Kee |
Author Affiliation: | Department of Biology, College of Education, Seoul National University |
Title: | The isolation and characterization of chlorophyll-protein complexes in thykaloid membranes from Zoysia japonica and Spinach oleracea |
Source: | Korean Journal of Turfgrass Science. Vol. 4, No. 1, 1990, p. 12-23 [271-282]. |
Publishing Information: | Korea: Turfgrass Society of Korea |
# of Pages: | 12 |
Keywords: | TIC Keywords: Light-harvesting complex; Thylakoids; Zoysia Japonica; Spinacia oleracea; Cell membranes
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Abstract/Contents: | "The chlorophyll-protein complexes were separated from thylakoid membranes of Spinach oleracea and Zoysia japonica by two gel systems of LiDodSO4-PAGE and LiDodSo4/Urea-PAGE under nondenaturing conditions. Seven chlorophyll-protein complexes of CPI*, CPI, CPII*, CP47, CP43, CP29, and CPII were fractionated from both S, oleracea and Z japonica by LiDodSo4-PAGE, CPI, CP47 and CP43 contained more chlorophyll a than chlorophyll b. The patterns of their absorption spectra at room temperature were similar to that of chlorophyll a, judging by their UV-spedtroscopy. On the other hand, CPII* and CPII contained approximately equimolar quantities of chlorophyll a and b. Additional five chlorophyll-protein complexes not separated in the LiDodSO4-PAGE system were electrophoretically isolated from both S, oleracea and Z japonica by LiDodSO4/Urea-PAGE. The chlorophyll-protein complex just above LHCII in the gel appears CCII-RC separated recently. 23 kDa and 20 kDa cho-protein complexes is probably LHCIa and LHCIb as judged from their molecular weight. Two novel chlorphyll-protein complexes designated "CP17" and "CP16" were fractionate by this gel system. Their molecular weights respectively. Although the stoichiometry of their components and their roles in thylakoid membranes are not apparant, It is thought that they are another kinds of LHCI." |
Language: | Korean |
References: | 37 |
Note: | Abstract appears in English Pictures, b/w Figures Tables Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Kim, B.-K., and N.-K. Chang. 1990. The isolation and characterization of chlorophyll-protein complexes in thykaloid membranes from Zoysia japonica and Spinach oleracea. (In Korean, with English abstract.) Korean Journal of Turfgrass Science. 4(1):p. 12-23 [271-282]. |
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| Web URL(s): http://img.kisti.re.kr/soc_img/society//tsk/JDHHBF/1990/v4n1/JDHHBF_1990_v4n1_12.pdf Last checked: 01/28/2013 Requires: PDF Reader |
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