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| Web URL(s): | http://img.kisti.re.kr/soc_img/society//tsk/JDHHBF/1991/v5n1/JDHHBF_1991_v5n1_11.pdf
Last checked: 01/28/2013
Requires: PDF Reader |
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| Publication Type:
| Refereed |
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| Author(s): | Park, Jae-Bok;
Kim, Young-Hoo;
Lee, Su-Young |
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| Author Affiliation: | Department of Environmental Horticulture, Seoul City University |
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| Title: | Studies on the idenfification of turfgrass by electrophoresis (SDS-PAGE, PAGIF) |
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| Source: | Korean Journal of Turfgrass Science. Vol. 5, No. 1, 1991, p. 11-22 [394-405]. |
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| Publishing Information: | Korea: Turfgrass Society of Korea |
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| # of Pages: | 12 |
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| Keywords: | TIC Keywords: Cultivar identification; Electrophoresis; Polyacrylamide gels; SDS-PAGE; Proteins
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| Abstract/Contents: | "This experiment was executed to investigate the possibility of the application of taxonomic method through the isoelectric focusing with polyacrylamide gel and sodium dodecyl sulfate-polyacrylamide gel electrophoresis with seeds in the identification of turfgrass. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to investigate the pattern of seed proteins which were extracted from 18 cultivars of cool season turfgrass and 4 cultivars of warm season turfgrass. The isoelectric focusing with polyacrylamide gel was used to investigate the activity of the three isozymes of esterase, peroxidase, and phosphoglucose isomerase which were extracted from 18 cultivars of cool season turfgrass and 4 cultivars of warm season turfgrass. The results were summarized as follows: 1. The difference of the patterns of seed proteins was observed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The identification of intra-genus was easily detected. 2. The three isozymes of esterase, peroxidase and phosphoglucose isomerase were investigated through isoelectric focusing with polyacrylamide gel. As a result, esterase was most effective among three isozymes in the identification of turfgrass cultivars. 3. In the past cultivar identification was primarily based on visual morphological characters, but there was a lot of difficulty. If we should use electrophoresis, we will be able to identify turfgrass cultivars more effectively." |
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| Language: | Korean |
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| References: | 20 |
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| Note: | Abstract appears in English
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Park, J.-B., Y.-H. Kim, and S.-Y. Lee. 1991. Studies on the idenfification of turfgrass by electrophoresis (SDS-PAGE, PAGIF). (In Korean, with English abstract.) Korean Journal of Turfgrass Science. 5(1):p. 11-22 [394-405]. |
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| Web URL(s):
http://img.kisti.re.kr/soc_img/society//tsk/JDHHBF/1991/v5n1/JDHHBF_1991_v5n1_11.pdf
Last checked: 01/28/2013
Requires: PDF Reader |
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MSU catalog number: b5472572a |
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