Full TGIF Record # 128472
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Web URL(s):http://img.kisti.re.kr/soc_img/society//tsk/JDHHBF/2001/v15n1/JDHHBF_2001_v15n1_9.pdf
    Last checked: 01/28/2013
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Author(s):Lim, Sun-Hyung; Byung-Chorl, Kang; Yong, Namgung; Hong-Gyun, Shin
Author Affiliation:Turfgrass and Environment Research Institute, Samsung Everland Incorporated Research Institute, Samsung Everland Incorporated
Title:Establishment of transformation systems of zoysiagrass by particle bombardment
Source:Korean Journal of Turfgrass Science. Vol. 15, No. 1, 2001, p. 9-14 [275-280].
# of Pages:6
Publishing Information:Korea: Turfgrass Society of Korea
Keywords:TIC Keywords: Calli; Regeneration; Zoysia; Particle bombardment; DNA; 2,4-D; Cytokinins
Cultivar Names:Zenith
Abstract/Contents:"Callus formation and plant regeneration from the seeds of zoysiagrass cv. Zenith was tested on MS basal medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid(2,4-D) and of several cytokinins. A concentration of 1 mg/L 2,4-D on medium stimulated callus formation. In the presence of 5 mg/L 2,4-D, addition of 1 mg/L kinetin significantly enhanced callus formation and plant regeneration over 2,4-D alone. To transfer foreign DNA into turfgrass, parameters for the bombardment of embryogenic callus with the particle bombardment were partially optimized using transient expression assay of a chimeric β -glucuronidase(GUS) gene driven by the CaMV 35S promoter. GUS gene was strongly expressed at helium pressure 1,100 psi and 6~9 cm target distance."
Note:Abstract appears in English
See Also:Other items relating to: 2, 4 - D in Turf
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Lim, S. -H., K. Byung-Chorl, N. Yong, and S. Hong-Gyun. 2001. Establishment of transformation systems of zoysiagrass by particle bombardment. (In Korean, with English abstract.) Korean Journal of Turfgrass Science. 15(1):p. 9-14 [275-280].
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    Last checked: 01/28/2013
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