| |
DOI: | 10.2135/cropsci2006.12.0781 |
Web URL(s): | https://dl.sciencesocieties.org/publications/cs/pdfs/47/5/2162 Last checked: 11/16/2016 Requires: PDF Reader Access conditions: Item is within a limited-access website https://dl.sciencesocieties.org/publications/cs/articles/47/5/2162 Last checked: 11/16/2016 Access conditions: Item is within a limited-access website |
Publication Type:
| Refereed |
Author(s): | Patton, Aaron J.;
Cunningham, Suzanne M.;
Volenec, Jeffrey J.;
Reicher, Zachary J. |
Author Affiliation: | Patton: Department of Horticulture, University of Arkansas, Fayetteville, Arkansas; Cunningham, Volenec, and Reicher: Department of Agronomy, Purdue University, West Lafayette, Indiana |
Title: | Differences in freeze tolerance of zoysiagrasses: I. Role of proteins |
Section: | Turfgrass science Other records with the "Turfgrass science" Section
|
Source: | Crop Science. Vol. 47, No. 5, September/October 2007, p. 2162-2169. |
Publishing Information: | Madison, WI: Crop Science Society of America |
# of Pages: | 8 |
Related Web URL: | https://dl.sciencesocieties.org/publications/cs/abstracts/47/5/2162 Last checked: 11/16/2016 Notes: Abstract only |
Keywords: | TIC Keywords: Cold resistance; Zoysia; Winter injury; Proteins; Genetic variability; Peptides; Cold acclimation; Photoperiod; SDS-PAGE; Dehydrins
|
Abstract/Contents: | "Genotypic variation in winter injury exists among zoysiagrasses (Zoysia spp.), but the physiological basis for these differences is not understood. Our objective was to determine the relationships between protein accumulation, polypeptide composition, and freeze tolerance of zoysiagrass. Thirteen genotypes of zoysiagrass with contrasting cold hardiness were identified. Cold acclimation was induced with 4 wk of 8/2°C day/night cycles and a 10-h photoperiod of 300 µmol m-2 s-11. Rhizomes and stolons of zoysiagrass were harvested from nonacclimated and cold-acclimated plants and used for protein analysis. Protein composition was analyzed using sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting with an antidehydrin polyclonal antibody. Buffer-soluble protein concentrations were higher among cold-acclimated (7.3 g kg-1 dry wt.) than nonacclimated (5.1 g kg-1 dry wt.) plants. The SDS-PAGE analysis indicated few differences in polypeptide composition among genotypes irrespective of cold acclimation. Immunoblotting indicated that dehydrin polypeptides (23 and 25 kDa) increased during cold acclimation. Abundance of the 23-kDa dehydrin polypeptide was positively associated (r2 = 0.41) with genetic variation in freezing tolerance. Our results suggest that dehydrins are associated with zoysiagrass cold acclimation, but that only the 23-kDa dehydrin plays a role in improving freeze tolerance." |
Language: | English |
References: | 54 |
See Also: | See also related article "Differences in freeze tolerance of zoysiagrasses: II. Carbohydrate and proline accumulation" Crop Science, 47(5) September/October 2007, p. 2170-2181 R=128761 R=128761 |
Note: | Tables Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Patton, A. J., S. M. Cunningham, J. J. Volenec, and Z. J. Reicher. 2007. Differences in freeze tolerance of zoysiagrasses: I. Role of proteins. Crop Sci. 47(5):p. 2162-2169. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=128759 |
| If there are problems with this record, send us feedback about record 128759. |
| Choices for finding the above item: |
| DOI: 10.2135/cropsci2006.12.0781 |
| Web URL(s): https://dl.sciencesocieties.org/publications/cs/pdfs/47/5/2162 Last checked: 11/16/2016 Requires: PDF Reader Access conditions: Item is within a limited-access website https://dl.sciencesocieties.org/publications/cs/articles/47/5/2162 Last checked: 11/16/2016 Access conditions: Item is within a limited-access website |
| MSU catalog number: SB 183 .C7 |
| Find from within TIC: Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |