Full TGIF Record # 130844
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DOI:10.2135/cropsci2007.04.2430-2436
Web URL(s):https://dl.sciencesocieties.org/publications/cs/pdfs/47/6/2430
    Last checked: 11/16/2016
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https://dl.sciencesocieties.org/publications/cs/articles/47/6/2430
    Last checked: 11/16/2016
    Access conditions: Item is within a limited-access website
Publication Type:
i
Refereed
Author(s):Luciani, Gabriela; Altpeter, Fredy; Yactayo-Chang, Jessica; Zhang, Hangning; Gallo, Maria; Meagher, Robert L.; Wofford, David
Author Affiliation:Luciani, Altpeter, Yactayo-Change, Zhang, Gallo, and Wofford: Agronomy Department, Genetics Institute, Plant Molecular and Cellular Biology Program, Institute of Food and Agricultural Sciences, University of Florida, ; Meagher: United States Department of Agriculture, Agricultural Research Service, Center for Medical, Agricultural, and Veterinary Entomology, Gainsville, Florida
Title:Expression of cry1Fa in bahiagrass enhances resistance to fall armyworm
Section:Genomics, molecular genetics & biotechnology
Other records with the "Genomics, molecular genetics & biotechnology" Section
Source:Crop Science. Vol. 47, No. 6, November/December 2007, p. 2430-2436.
Publishing Information:Madison, WI: Crop Science Society of America
# of Pages:7
Related Web URL:https://dl.sciencesocieties.org/publications/cs/abstracts/47/6/2430
    Last checked: 11/16/2016
    Notes: Abstract only
Keywords:TIC Keywords: Paspalum notatum; Spodoptera frugiperda; Scapteriscus; Bacillus thuringiensis; Insect resistance; Transgene expression
Abstract/Contents:"Bahiagrass (Paspalum notatum var. saurae) is the predominant forage grass in Florida and in other subtropical regions. To improve pest resistance against fall armyworm [Spodoptera frugiperda (J. E. Smith)], an optimized cry1Fa gene encoding a δ-endotoxin from Bacillus thuringiensis was synthesized, subcloned under the transcriptional control of the constitutive ubi1 promoter, and introduced into the bahiagrass cultivar Tifton 9 by particle bombardment. Three transgenic bahiagrass lines were generated using minimal transgene expression cassettes without vector backbone. Southern blot analyses showed independent cry1Fa transgene integration patterns for the three lines. Transcripts of cry1Fa were detected in all three transgenic lines by reverse transcriptase polymerase chain reaction. Cry1Fa protein was detected in two lines by immuno-chromatography and quantitative Cry1Fa enzyme linked immunosorbent assay (ELISA). The Cry1Fa ELISA also indicated stable cry1Fa transgene expression in vegetative progeny plants of both lines. Cry1Fa expression levels correlated well to resistance levels determined by insect bioassays. An average mortality rate of 83% was observed when neonate larvae of fall armyworm were fed with transgenic leaves of the highest cry1Fa expressing line. These results indicate that minimal expression cassette technology supports stable and high level expression of cry1Fa in bahiagrass which can control fall armyworm, a devastating pest of forage grasses."
Language:English
References:43
Note:Pictures, b/w
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Luciani, G., F. Altpeter, J. Yactayo-Chang, H. Zhang, M. Gallo, R. L. Meagher, et al. 2007. Expression of cry1Fa in bahiagrass enhances resistance to fall armyworm. Crop Sci. 47(6):p. 2430-2436.
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DOI: 10.2135/cropsci2007.04.2430-2436
Web URL(s):
https://dl.sciencesocieties.org/publications/cs/pdfs/47/6/2430
    Last checked: 11/16/2016
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
https://dl.sciencesocieties.org/publications/cs/articles/47/6/2430
    Last checked: 11/16/2016
    Access conditions: Item is within a limited-access website
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