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| Web URL(s): | http://img.kisti.re.kr/soc_img/society//tsk/JDHHBF/2004/v18n4/JDHHBF_2004_v18n4_211.pdf
Last checked: 01/28/2013
Requires: PDF Reader |
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| Publication Type:
| Refereed |
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| Author(s): | Lim, Sun-Hyung;
Kang, Byung-Chorl;
Shin, Hong-Kyun |
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| Author Affiliation: | Turfgrass and Environmental Research Institute, Samsung Everland, Kunpo, Korea |
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| Title: | Herbicide resistant turfgrass(Zoysia japonica cv. 'Zenith') plants by particle bombardment-mediated transformation |
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| Source: | Korean Journal of Turfgrass Science. Vol. 18, No. 4, 2004, p. 211-219. |
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| Publishing Information: | Korea: Turfgrass Society of Korea |
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| # of Pages: | 9 |
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| Keywords: | TIC Keywords: Herbicide resistance; Zoysia Japonica; Particle bombardment; Genetic transformation; Transgene expression
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| Cultivar Names: | Zenith |
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| Abstract/Contents: | "Transgenic zoysiagrass (Zoysia japonica cv. Zenith) plants have been obtained by particle bombardment of embryogenic callus with the plasmid pSMABuba, which contains hygromycin resistance (hpt) and bialaphos resistance (bar) genes. Parameters on DNA delivery efficiency of the particle bombardment were partially optimized suing transient expression assay of a chimeric β-glucuronidase (gusA) gene driven by the CaMV 35S promoter. Stably transformed zoysiagrass plants were recovered with a selction scheme using hygromycin. Transgenic zoysiagrass plants were confirmed by PCR analysis with specific primer for bar gene. Expression of the transgene in transformed zoysiagrass plants was demonstrated by Reverse transcriptase (RT)-PCR analysis. All the tested transgenic plants showed herbicide BastaR resistance at the field application rate of 0.1%-0.3%." |
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| Language: | English |
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| References: | 26 |
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| Note: | Abstract appears in English
Pictures, b/w |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Lim, S.-H., B.-C. Kang, and H.-K. Shin. 2004. Herbicide resistant turfgrass(Zoysia japonica cv. 'Zenith') plants by particle bombardment-mediated transformation. (In English, with English abstract.) Korean Journal of Turfgrass Science. 18(4):p. 211-219. |
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| Web URL(s):
http://img.kisti.re.kr/soc_img/society//tsk/JDHHBF/2004/v18n4/JDHHBF_2004_v18n4_211.pdf
Last checked: 01/28/2013
Requires: PDF Reader |
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MSU catalog number: b5472572a |
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