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| DOI: | 10.1614/WS-07-057.1 |
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| Web URL(s): | http://www.bioone.org/perlserv/?request=get-document&doi=10.1614%2FWS-07-057.1
Last checked: 04/15/08
Requires: PDF Reader
Access conditions: Item is within a limited access website |
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| Publication Type:
| Refereed |
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| Author(s): | Simarmata, Marulak;
Penner, Donald |
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| Author Affiliation: | Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan |
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| Title: | The basis for glyphosate resistance in rigid ryegrass (Lolium rigidum) from California |
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| Section: | Physiology, chemistry, and biochemistry
Other records with the "Physiology, chemistry, and biochemistry" Section
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| Source: | Weed Science. Vol. 56, No. 2, March/April 2008, p. 181-188. |
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| Publishing Information: | Champaign, IL: Weed Science Society of America |
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| # of Pages: | 8 |
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| Keywords: | TIC Keywords: Glyphosate; Lolium rigidum; Herbicide resistance; Herbicide metabolism; Mutations; EPSP synthase; Metabolism
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| Abstract/Contents: | "The occurrence of glyphosate-resistant weeds has been reported after more than 20 yr of extensive use. Rigid ryegrass that evolved resistance to glyphosate was found in Australia and in California. Glyphosate-resistant rigid ryegrass plants were collected from northern California and selected through generations 8 and 5 to segregate the most resistant (R) and sensitive (S) biotypes. The eighth generation of R and the fifth generation of S biotypes survived 6.72 and died from 0.11 kg ae ha-1 glyphosate, respectively. The objectives of this study were to evaluate the role of metabolism in the observed resistance, to study the effect of glyphosate on the activity of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS; EC 2.5.1.19), and to characterize the EPSPS gene in R and S rigid ryegrass. Neither quantitative nor qualitative difference was observed in the metabolism of 14C-glyphosate between the biotypes. Activity of constitutive EPSPS decreased more significantly in the S than R biotype in the presence of 5, 50, 500, and 5,000 µM glyphosate. Inhibition of 50% (I50) of the EPSPS activity by glyphosate was more than 90-fold in S compared to R biotype. Decreased EPSPS sensitivity in the R biotype appeared to be a major contributor to glyphosate resistance in rigid ryegrass from California. Fragments of the EPSPS gene containing 1,320 nucleotides were isolated from mRNA of S and R biotypes. A single nucleotide mutation from cytosine (C) to thymine (T) was identified at nucleotide 301 of the truncated EPSPS gene of the R biotype. This mutation changed the amino acid code from proline (Pro) to serine (Ser), which was similar to that reported for the glyphosate-resistant goosegrass from Malaysia and correlated with glyphosate insensitivity of EPSPS." |
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| Language: | English |
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| References: | 25 |
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| Note: | Pictures, b/w
Figures
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Simarmata, M., and D. Penner. 2008. The basis for glyphosate resistance in rigid ryegrass (Lolium rigidum) from California. Weed Sci. 56(2):p. 181-188. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=134935 |
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| DOI: 10.1614/WS-07-057.1 |
| Web URL(s):
http://www.bioone.org/perlserv/?request=get-document&doi=10.1614%2FWS-07-057.1
Last checked: 04/15/08
Requires: PDF Reader
Access conditions: Item is within a limited access website |
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MSU catalog number: b2203399a |
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